Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products

被引：1
|
作者：
Qu, Meng [1 ,2 ]
Jiang, Yanhua [1 ,2 ]
Guo, Yingying [1 ,2 ]
Zhu, Wenjia [1 ,2 ]
Liu, Shufang [2 ,3 ]
Li, Na [1 ,2 ]
Li, Fengling [1 ,2 ]
Yao, Lin [1 ,2 ,4 ]
Tan, Zhijun [1 ,2 ]
Wang, Lianzhu [1 ,2 ]
机构：
[1] Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Qingdao, Peoples R China
[2] Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Qingdao, Peoples R China
[3] Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
[4] Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
来源：
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY | 2023年 / 32卷 / 10期
关键词：
Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene; LEPIDOCYBIUM-FLAVOBRUNNEUM; RUVETTUS-PRETIOSUS; IDENTIFICATION; GUIDELINES; SALMON; CHINA;
D O I：
10.1080/10498850.2023.2270217
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng mu L-1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg(-1). The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.
引用
收藏
页码：667 / 676
页数：10
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