Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products

被引：1

作者：

Qu, Meng ^{[1
,2
]}

Jiang, Yanhua ^{[1
,2
]}

Guo, Yingying ^{[1
,2
]}

Zhu, Wenjia ^{[1
,2
]}

Liu, Shufang ^{[2
,3
]}

Li, Na ^{[1
,2
]}

Li, Fengling ^{[1
,2
]}

Yao, Lin ^{[1
,2
,4
]}

Tan, Zhijun ^{[1
,2
]}

Wang, Lianzhu ^{[1
,2
]}

机构：

[1] Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Qingdao, Peoples R China

[2] Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Qingdao, Peoples R China

[3] Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China

[4] Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China

来源：

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY | 2023年 / 32卷 / 10期

关键词：

Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene; LEPIDOCYBIUM-FLAVOBRUNNEUM; RUVETTUS-PRETIOSUS; IDENTIFICATION; GUIDELINES; SALMON; CHINA;

D O I：

10.1080/10498850.2023.2270217

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng mu L-1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg(-1). The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.

引用

页码：667 / 676

页数：10

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