Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products

被引：1
|
作者：
Qu, Meng [1 ,2 ]
Jiang, Yanhua [1 ,2 ]
Guo, Yingying [1 ,2 ]
Zhu, Wenjia [1 ,2 ]
Liu, Shufang [2 ,3 ]
Li, Na [1 ,2 ]
Li, Fengling [1 ,2 ]
Yao, Lin [1 ,2 ,4 ]
Tan, Zhijun [1 ,2 ]
Wang, Lianzhu [1 ,2 ]
机构：
[1] Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Qingdao, Peoples R China
[2] Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Qingdao, Peoples R China
[3] Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
[4] Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
来源：
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY | 2023年 / 32卷 / 10期
关键词：
Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene; LEPIDOCYBIUM-FLAVOBRUNNEUM; RUVETTUS-PRETIOSUS; IDENTIFICATION; GUIDELINES; SALMON; CHINA;
D O I：
10.1080/10498850.2023.2270217
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng mu L-1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg(-1). The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.
引用
收藏
页码：667 / 676
页数：10
相关论文
共 50 条
  • [21] Comparison of standard polymerase chain reaction (PCR) with real-time pcr for meq gene of Marek's disease virus
    Krol, Katarzyna
    Baigent, Sue
    Nair, Venugopal
    Kozdrun, Wojciech
    Samorek-Salamonowicz, Elzbieta
    BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2007, 51 (01) : 3 - 7
  • [22] Conventional multiplex polymerase chain reaction (PCR) versus real-time PCR for species-specific meat authentication
    Edris, S.
    Mutwakil, M. H. Z.
    Abuzinadah, O. A.
    Mohammed, H. E.
    Ramadan, A.
    Gadalla, N. O.
    Shokry, A. M.
    Hassan, S. M.
    Shoaib, R. M.
    El-Domyati, F. M.
    Bahieldin, A.
    LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION, 2012, 9 (04): : 5831 - 5837
  • [23] Development of TaqMan real-time polymerase chain reaction for the detection and identification of Penicillium marneffei
    Pornprasert, Sakorn
    Praparattanapan, Jutarat
    Khamwan, Chantana
    Pawichai, Sudjai
    Pimsarn, Parichat
    Samleerat, Tanawan
    Leechanachai, Pranee
    Supparatpinyo, Khunchai
    MYCOSES, 2009, 52 (06) : 487 - 492
  • [24] Development of quantitative real-time polymerase chain reaction for duck enteritis virus DNA
    Yang, FL
    Jia, WX
    Yue, H
    Luo, W
    Chen, X
    Xie, Y
    Zen, W
    Yang, WQ
    AVIAN DISEASES, 2005, 49 (03) : 397 - 400
  • [25] Evaluation of a broad range real-time polymerase chain reaction (RT-PCR) assay for the diagnosis of septic synovitis in horses
    Elmas, Colette R.
    Koenig, Judith B.
    Bienzle, Dorothee
    Cribb, Nicola C.
    Cernicchiaro, Natalia
    Cote, Nathalie M.
    Weese, J. Scott
    CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2013, 77 (03): : 211 - 217
  • [26] Comparison of nested polymerase chain reaction (PCR), real-time PCR and viral culture for the detection of cytomegalovirus in subgingival samples
    Botero, J. E.
    Vidal, C.
    Contreras, A.
    Parra, B.
    ORAL MICROBIOLOGY AND IMMUNOLOGY, 2008, 23 (03): : 239 - 244
  • [27] A kinetic model of quantitative real-time polymerase chain reaction
    Mehra, S
    Hu, WS
    BIOTECHNOLOGY AND BIOENGINEERING, 2005, 91 (07) : 848 - 860
  • [28] Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR
    Hird, HJ
    Hold, GL
    Chisholm, J
    Reece, P
    Russell, VJ
    Brown, J
    Goodier, R
    MacArthur, R
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2005, 220 (5-6) : 633 - 637
  • [29] Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR
    H. J. Hird
    G. L. Hold
    J. Chisholm
    P. Reece
    V. J. Russell
    J. Brown
    R. Goodier
    R. MacArthur
    European Food Research and Technology, 2005, 220 : 633 - 637
  • [30] Development and Evaluation of a Multiplex Real-Time Polymerase Chain Reaction Procedure to Clinically Type Prevalent Salmonella enterica Serovars
    Munoz, Nelida
    Diaz-Osorio, Miguel
    Moreno, Jaime
    Sanchez-Jimenez, Miryan
    Cardona-Castro, Nora
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2010, 12 (02) : 220 - 225
12345