A Novel Hydroxyapatite/Vitamin B12 Nanoformula for Treatment of Bone Damage: Preparation, Characterization, and Anti-Arthritic, Anti-Inflammatory, and Antioxidant Activities in Chemically Induced Arthritic Rats

被引：7

作者：

Belal, Amany ^{[1
]}

Mahmoud, Rehab ^{[2
]}

Mohamed, Eman E. ^{[3
]}

Farghali, Ahmed ^{[4
]}

El-Ela, Fatma I. Abo ^{[5
]}

Gamal, Amr ^{[6
]}

Halfaya, Fatma Mohamed ^{[7
]}

Khaled, Esraa ^{[4
]}

Farahat, Abdelbasset A. ^{[8
,9
]}

Hassan, Ahmed H. E. ^{[10
,11
]}

Ghoneim, Mohammed M. ^{[12
]}

Taha, Mohamed ^{[4
]}

Zaky, Mohamed Y. ^{[3
,13
,14
]}

机构：

[1] Taif Univ, Coll Pharm, Dept Pharmaceut Chem, Taif 21944, Saudi Arabia

[2] Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

[3] Beni Suef Univ, Fac Sci, Zool Dept, Mol Physiol Div, Bani Suwayf 62511, Egypt

[4] Beni Suef Univ, Fac Postgrad Studies Adv Sci, Mat Sci & Nanotechnol Dept, Bani Suwayf 62511, Egypt

[5] Beni Suef Univ, Fac Vet Med, Dept Pharmacol, Bani Suwayf 62511, Egypt

[6] Beni Suef Univ, Fac Pharm, Dept Pharmaceut & Ind Pharm, Bani Suwayf 62511, Egypt

[7] Beni Suef Univ, Fac Vet Med, Dept Surg Anesthesiol & Radiol, Bani Suwayf 62511, Egypt

[8] Calif Northstate Univ, Pharmaceut Sci Program, 9700 W Taron Dr, Elk Grove, CA 95757 USA

[9] Mansoura Univ, Fac Pharm, Dept Pharmaceut Organ Chem, Mansoura 35516, Egypt

[10] Mansoura Univ, Fac Pharm, Dept Med Chem, Mansoura 35516, Egypt

[11] Kyung Hee Univ, Coll Pharm, Med Chem Lab, 26 Kyungheedae Ro, Seoul 02447, South Korea

[12] AlMaarefa Univ, Coll Pharm, Dept Pharm Practice, Ad Diriyah 13713, Saudi Arabia

[13] Linkoping Univ, Fac Med, Dept Oncol, S-58183 Linkoping, Sweden

[14] Linkoping Univ, Fac Med, Dept Biomed & Clin Sci, S-58183 Linkoping, Sweden

来源：

PHARMACEUTICALS | 2023年 / 16卷 / 04期

关键词：

bone damage; bone disability research; rheumatoid arthritis; nano-HAP; Vit B-12; anti-arthritic; antioxidant; anti-inflammatory; GROWTH-FACTOR-BETA; RHEUMATOID-ARTHRITIS; OSTEOINDUCTIVE BIOMATERIALS; EXPRESSION; METALLOPROTEINASES; CYTOKINES; ALPHA; CELL;

D O I：

10.3390/ph16040551

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The usage of nanomaterials for rheumatoid arthritis (RA) treatment can improve bioavailability and enable selective targeting. The current study prepares and evaluates the in vivo biological effects of a novel hydroxyapatite/vitamin B-12 nanoformula in Complete Freund's adjuvant-induced arthritis in rats. The synthesized nanoformula was characterized using XRD, FTIR, BET analysis, HERTEM, SEM, particle size, and zeta potential. We synthesized pure HAP NPs with 71.01% loading weight percentages of Vit B12 and 49 mg/g loading capacity. Loading of vitamin B-12 on hydroxyapatite was modeled by Monte Carlo simulation. Anti-arthritic, anti-inflammatory, and antioxidant effects of the prepared nanoformula were assessed. Treated arthritic rats showed lower levels of RF and CRP, IL-1 beta, TNF-alpha, IL-17, and ADAMTS-5, but higher IL-4 and TIMP-3 levels. In addition, the prepared nanoformula increased GSH content and GST antioxidant activity while decreasing LPO levels. Furthermore, it reduced the expression of TGF-beta mRNA. Histopathological examinations revealed an improvement in joint injuries through the reduction of inflammatory cell infiltration, cartilage deterioration, and bone damage caused by Complete Freund's adjuvant. These findings indicate that the anti-arthritic, antioxidant, and anti-inflammatory properties of the prepared nanoformula could be useful for the development of new anti-arthritic treatments.

引用

页数：19

共 83 条

[31] Nano-hydroxyapatite improves intestinal absorption of acetazolamide (BCS Class IV drug)-but how? [J].