Metabolomics Integration in Assisted Reproductive Technologies for Enhanced Embryo Selection beyond Morphokinetic Analysis

被引:4
作者
Pinto, Soraia [1 ]
Guerra-Carvalho, Barbara [2 ]
Crisostomo, Luis [3 ]
Rocha, Antonio [4 ]
Barros, Alberto [1 ,5 ,6 ]
Alves, Marco G. [7 ]
Oliveira, Pedro F. [2 ]
D'Hooghe, Thomas
机构
[1] Ctr Reprod Genet Alberto Barros, P-4100012 Porto, Portugal
[2] Univ Aveiro, Dept Chem, LAQV REQUIMTE, P-3810193 Aveiro, Portugal
[3] Univ Turku, Inst Biomed, Turku 20014, Finland
[4] Univ Porto, Inst Biomed Sci Abel Salazar ICBAS, Ctr Estudos Ciencia Anim, CECA ICETA, P-4200135 Porto, Portugal
[5] Univ Porto, i3S Inst Invest & Inovacao Saude, P-4200135 Porto, Portugal
[6] Univ Porto, Fac Med, Dept Pathol, P-4200319 Porto, Portugal
[7] Univ Aveiro, Inst Biomed iBiMED, Dept Med Sci, P-3810193 Aveiro, Portugal
关键词
embryo quality evaluation; morphokinetic assessment; embryo metabolomics; pyruvate; glutamine; IN-VITRO; NONINVASIVE ASSESSMENT; ENERGY-METABOLISM; HUMAN OOCYTES; MOUSE; OBESITY; MITOCHONDRIA; INFERTILITY; OVERWEIGHT; CULTURE;
D O I
10.3390/ijms25010491
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Embryo quality evaluation during in vitro development is a crucial factor for the success of assisted reproductive technologies (ARTs). However, the subjectivity inherent in the morphological evaluation by embryologists can introduce inconsistencies that impact the optimal embryo choice for transfer. To provide a more comprehensive evaluation of embryo quality, we undertook the integration of embryo metabolomics alongside standardized morphokinetic classification. The culture medium of 55 embryos (derived from 21 couples undergoing ICSI) was collected at two timepoints (days 3 and 5). Samples were split into Good (n = 29), Lagging (n = 19), and Bad (n = 10) according to embryo morphokinetic evaluation. Embryo metabolic performance was assessed by monitoring the variation in specific metabolites (pyruvate, lactate, alanine, glutamine, acetate, formate) using 1H-NMR. Adjusted metabolite differentials were observed during the first 3 days of culture and found to be discriminative of embryo quality at the end of day 5. Pyruvate, alanine, glutamine, and acetate were major contributors to this discrimination. Good and Lagging embryos were found to export and accumulate pyruvate and glutamine in the first 3 days of culture, while Bad embryos consumed them. This suggests that Bad embryos have less active metabolic activity than Good and Lagging embryos, and these two metabolites are putative biomarkers for embryo quality. This study provides a more comprehensive evaluation of embryo quality and can lead to improvements in ARTs by enabling the selection of the best embryos. By combining morphological assessment and metabolomics, the selection of high-quality embryos with the potential to result in successful pregnancies may become more accurate and consistent.
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页数:18
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