The phenylalanine-28 is crucial for black carp RIG-I mediated antiviral signaling

被引:2
作者
Liu, Ji [1 ,2 ]
He, Yixuan [1 ]
Miao, Yujia [1 ]
Dai, Chushan [1 ]
Yan, Jun [1 ,2 ]
Liu, Meiling [2 ]
Zou, Jun [3 ]
Feng, Hao [1 ]
机构
[1] Hunan Normal Univ, Coll Life Sci, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China
[2] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Peoples R China
[3] Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Innate immunity; RIG-I; Interferon; Black carp; PATTERN-RECOGNITION RECEPTORS; INNATE IMMUNE-RESPONSE; STRUCTURAL INSIGHTS; RNA RECOGNITION; ACTIVATION; MAVS; MDA5; IDENTIFICATION; HELICASES; VARIANT;
D O I
10.1016/j.dci.2023.104917
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Retinoic acid-inducible gene I (RIG-I) functions as a cytosolic sensor to recognize RNA products of the invading microorganisms and induce the production of type I interferons(IFNs). In this study, two RIG-I variants, named as bcRIG-Ia and bcRIG-Ib, were characterized in black carp (Mylopharyngodon piceus) respectively. RNA pulldown assay revealed that both bcRIG-Ia and bcRIG-Ib could bind to synthetic poly(I:C) and the RD domain was crucial for RNA binding of these two molecules. However, over-expression of bcRIG-Ib, but not bcRIG-Ia, induced the transcription of IFN promoter, and led to the improved antiviral activity against both spring viremia of carp virus (SVCV) and grass carp reovirus (GCRV). And knockdown of bcRIG-I dampened the transcription of bcViperin and bcIFNb in host cells. Truncation mutation and site mutation analysis identified that phenylalanine (F)- 28 was crucial for bcRIG-Ib oligomerization and its mediated IFN signaling. Interestingly, F28 was conserved among teleost RIG-Is and site mutation analysis revealed that F28 was essential for RIG-I mediated IFN signaling in the cyprinid fish. Thus, our study concludes that F28 is crucial for black carp RIG-I mediated antiviral signaling and suggests F28 is also essential for the activation of IFN signaling by RIG-Is from other teleost fish.
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页数:13
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共 35 条
[1]   Pathogen recognition and innate immunity [J].
Akira, S ;
Uematsu, S ;
Takeuchi, O .
CELL, 2006, 124 (04) :783-801
[2]   Newly described pattern recognition receptors team up against intracellular pathogens [J].
Broz, Petr ;
Monack, Denise M. .
NATURE REVIEWS IMMUNOLOGY, 2013, 13 (08) :551-565
[3]   Expression and Functional Characterization of the RIG-I-Like Receptors MDA5 and LGP2 in Rainbow Trout (Oncorhynchus mykiss) [J].
Chang, Mingxian ;
Collet, Bertrand ;
Nie, Pin ;
Lester, Katherine ;
Campbell, Scott ;
Secombes, Christopher J. ;
Zou, Jun .
JOURNAL OF VIROLOGY, 2011, 85 (16) :8403-8412
[4]   RIG-I specifically mediates group II type I IFN activation in nervous necrosis virus infected zebrafish cells [J].
Chen, Hsu-Yu ;
Liu, Wangta ;
Wu, Sung-Yu ;
Chiou, Pinwen Peter ;
Li, Yen-Hsing ;
Chen, Yen-Chun ;
Lin, Gen-Hwa ;
Lu, Ming-Wei ;
Wu, Jen-Leih .
FISH & SHELLFISH IMMUNOLOGY, 2015, 43 (02) :427-435
[5]   Functional Characterizations of RIG-I to GCRV and Viral/Bacterial PAMPs in Grass Carp Ctenopharyngodon idella [J].
Chen, Lijun ;
Su, Jianguo ;
Yang, Chunrong ;
Peng, Limin ;
Wan, Quanyuan ;
Wang, Lan .
PLOS ONE, 2012, 7 (07)
[6]   Eat me and "don't eat me" signals govern the innate immune response and tissue repair in the CNS: emphasis on the critical role of the complement system [J].
Elward, K ;
Gasque, P .
MOLECULAR IMMUNOLOGY, 2003, 40 (2-4) :85-94
[7]   Roles of RIG-1 N-terminal tandem CARD and splice variant in TRIM25-mediated antiviral signal transduction [J].
Gack, Michaela U. ;
Kirchhofer, Axel ;
Shin, Young C. ;
Inn, Kyung-Soo ;
Liang, Chengyu ;
Cui, Sheng ;
Myong, Sua ;
Ha, Taekjip ;
Hopfner, Karl-Peter ;
Jung, Jae U. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (43) :16743-16748
[8]   TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity [J].
Gack, Michaela U. ;
Shin, Young C. ;
Joo, Chul-Hyun ;
Urano, Tomohiko ;
Liang, Chengyu ;
Sun, Lijun ;
Takeuchi, Osamu ;
Akira, Shizuo ;
Chen, Zhijian ;
Inoue, Satoshi ;
Jung, Jae U. .
NATURE, 2007, 446 (7138) :916-U2
[9]   MAVS Forms Functional Prion-like Aggregates to Activate and Propagate Antiviral Innate Immune Response [J].
Hou, Fajian ;
Sun, Lijun ;
Zheng, Hui ;
Skaug, Brian ;
Jiang, Qiu-Xing ;
Chen, Zhijian J. .
CELL, 2011, 146 (03) :448-461
[10]   Identification of a novel RIG-I isoform and its truncating variant in Japanese eel, Anguilla japonica [J].
Huang, B. ;
Wang, Z. X. ;
Zhang, C. ;
Zhai, S. W. ;
Han, Y. S. ;
Huang, W. S. ;
Nie, P. .
FISH & SHELLFISH IMMUNOLOGY, 2019, 94 :373-380