The Phosphatase VdPtc3 Regulates Virulence in Verticillium dahliae by Interacting with VdAtg1

被引：2
作者：
Liu, Tao [1 ]
Qin, Jun [1 ]
Shang, Wenjing [1 ]
Chen, Jieyin [2 ,3 ]
Subbarao, Krishna. V. V. [4 ]
Hu, Xiaoping [1 ]
机构：
[1] Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Xianyang, Peoples R China
[2] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
[3] Chinese Acad Agr Sci, Western Agr Res Ctr, Changji, Peoples R China
[4] Univ Calif Davis, Dept Plant Pathol, Agr Res Stn, Salinas, CA USA
来源：
PHYTOPATHOLOGY | 2023年 / 113卷 / 06期
关键词：
melanin; microsclerotia; VdAtg1; VdPtc3; Verticillium wilt; virulence; ACTIVATED PROTEIN-KINASE; OSMOTIC-STRESS RESPONSE; MICROSCLEROTIA FORMATION; NEUROSPORA-CRASSA; GENES; COLONIZATION; PATHOGENICITY; RESISTANT; PATHWAYS; MELANINS;
D O I：
10.1094/PHYTO-09-22-0320-R
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Type 2C protein phosphatases regulate various biological processes in eukaryotes. However, their functions in Verticillium dahliae have not been characterized. In this study, homologs VdPtc1, VdPtc3, VdPtc5, VdPtc6, and VdPtc7 were identified in V. dahliae on the basis of homologous comparison with those in Saccharomyces cerevisiae. VdPtc2 and VdPtc4 are missing in the genome of the V. dahliae XJ592 strain. VdPtc3 is the homolog of Ptc2, Ptc3, and Ptc4 proteins in S. cerevisiae, implying that VdPtc3 may play versatile functions in V. dahliae. VdPtc3 promoted conidium development, melanin, and microsclerotium formation in V. dahliae. The Delta VdPtc3 strains showed increased sensitivity to NaCl and sorbitol and augmented the phosphorylation of p38 mitogen-activated protein kinase homolog Hog1 induced by osmotic stress. Besides, the Delta VdPtc3 strains also showed milder Verticillium wilt symptom on cotton. Furthermore, VdPtc3 interacts with VdAtg1, which modulates melanin and microsclerotium formation, as well as pathogenicity.
引用
收藏
页码：1048 / 1057
页数：10
相关论文
共 52 条
  • [11] Integrative proteomics and biochemical analyses define Ptc6p as the Saccharomyces cerevisiae pyruvate dehydrogenase phosphatase
    Guo, Xiao
    Niemi, Natalie M.
    Coon, Joshua J.
    Pagliarini, David J.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (28) : 11751 - 11759
  • [12] Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function
    Guo, Xiao
    Niemi, Natalie M.
    Hutchins, Paul D.
    Condon, Samson G. F.
    Jochem, Adam
    Ulbrich, Arne
    Higbee, Alan J.
    Russell, Jason D.
    Senes, Alessandro
    Coon, Joshua J.
    Pagliarini, David J.
    [J]. CELL REPORTS, 2017, 18 (02): : 307 - 313
  • [13] MAP kinase pathways in the yeast Saccharomyces cerevisiae
    Gustin, MC
    Albertyn, J
    Alexander, M
    Davenport, K
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) : 1264 - +
  • [14] Assay of protein kinases using radiolabeled ATP: a protocol
    Hastie, C. James
    McLauchlan, Hilary J.
    Cohen, Philip
    [J]. NATURE PROTOCOLS, 2006, 1 (02) : 968 - 971
  • [15] The dark side of the mycelium: Melanins of phytopathogenic fungi
    Henson, JM
    Butler, MJ
    Day, AW
    [J]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 1999, 37 : 447 - 471
  • [16] Whole Genome Wide Expression Profiles on Germination of Verticillium dahliae Microsclerotia
    Hu, Dongfang
    Wang, Chunsheng
    Tao, Fei
    Cui, Qian
    Xu, Xiangming
    Shang, Wenjing
    Hu, Xiaoping
    [J]. PLOS ONE, 2014, 9 (06):
  • [17] An optimized method for in vitro production of Verticillium dahliae microsclerotia
    Hu, Xiaoping
    Bai, Yinwen
    Chen, Ting
    Hu, Dongfang
    Yang, Jiarong
    Xu, Xiangming
    [J]. EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2013, 136 (02) : 225 - 229
  • [18] Signaling - 2000 and beyond
    Hunter, T
    [J]. CELL, 2000, 100 (01) : 113 - 127
  • [19] Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1
    Jacoby, T
    Flanagan, H
    Faykin, A
    Seto, AG
    Mattison, C
    Ota, I
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (28) : 17749 - 17755
  • [20] A Type 2C Protein Phosphatase FgPtc3 Is Involved in Cell Wall Integrity, Lipid Metabolism, and Virulence in Fusarium graminearum
    Jiang, Jinhua
    Yun, Yingzi
    Yang, Qianqian
    Shim, Won-Bo
    Wang, Zhengyi
    Ma, Zhonghua
    [J]. PLOS ONE, 2011, 6 (09):
123456