Functional characterization of two corticotropin-releasing hormone receptors in Larimichthys crocea

被引：0

作者：

Liu, Xue ^{[1
]}

Feng, Jiaqian ^{[1
]}

Jiang, Zhijing ^{[1
]}

Zhang, Guangbo ^{[1
]}

Xu, Xiuwen ^{[1
]}

Wang, Jixiu ^{[1
]}

Yang, Jingwen ^{[1
]}

Wang, Tianming ^{[1
]}

机构：

[1] Zhejiang Ocean Univ, Marine Sci & Technol Coll, Natl Engn Lab Marine Germplasm Resources Explorat, Zhoushan, Zhejiang, Peoples R China

来源：

FRONTIERS IN MARINE SCIENCE | 2023年 / 10卷

基金：

中国国家自然科学基金;

关键词：

stress; reproduction; neuroendocrine; Larimichthys crocea; ovary; corticotropin-releasing hormone receptor; IMPAIRED STRESS-RESPONSE; FISH UROTENSIN-I; CRH-RECEPTOR; PHOSPHOLIPASE-C; BINDING PROTEIN; EXPRESSION; PEPTIDE; ACTIVATION; EVOLUTION; UROCORTIN;

D O I：

10.3389/fmars.2023.1184792

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In the neuroendocrine system, corticotropin-releasing hormone (CRH) plays an important role in the hypothalamic-pituitary-adrenal/interrenal (HPA/HPI) axis. It exerts its effects by activating CRHRs, which belong to the class B G protein-coupled receptor family. Two characteristic genes of CRHR1 subtypes in the Larimichthys crocea genome were identified: LcCRHR1-1 and LcCRHR1-2. Alignments indicated that they were highly homologous to known and validated teleost CRHR1s. The CDS sequences of the two receptors were cloned into the pEGFP-N1 plasmid, and membrane localization of the fusion expressing LcCRHR1-1-EGFP and LcCRHR1-2-EGFP was revealed in HEK293 cells. Treatment with LcCRH could lead to two receptors internalization and trigger a significant increase in the secondary messenger cAMP and Ca2+ and mitogen-activated protein kinase phosphorylation in an LcCRH dose-dependent manner. Based on quantitative real-time PCR, LcCRHR1s were expressed in all examined tissues and highly expressed in the brain and ovaries. Furthermore, immunohistochemical findings showed the specific localization of CRHR1s in ovarian follicle cells. Collectively, our study identified two CRH receptors in L. crocea and suggested that the CRH/CRHR1 system is potentially involved in the neuroendocrine regulation of reproduction in this marine fish.

引用

页数：14

共 78 条

[1] Characterization of three corticotropin-releasing factor receptors in catfish: A novel third receptor is predominantly expressed in pituitary and urophysis [J].