Analyzing the Material Basis of Anti-RSV Efficacy of Lonicerae japonicae Flos Based on the PK-PD Model

被引:2
作者
Liang, Yuting [1 ]
Liu, Mingjun [1 ]
Wang, Yanghai [1 ]
Liu, Lu [1 ]
Gao, Yan [1 ,2 ,3 ]
机构
[1] Shandong Univ Tradit Chinese Med, Inst Pharmaceut Res, Jinan 250355, Peoples R China
[2] Pharmaceut Tradit Chinese Med, High Level Tradit Chinese Med Key Disciplines Stat, Jinan 250355, Peoples R China
[3] Collaborat Innovat Ctr Ecol Protect & High Qual De, Jinan 250355, Peoples R China
来源
MOLECULES | 2023年 / 28卷 / 18期
关键词
Lonicerae japonicae Flos; respiratory syncytial virus; effective ingredient; UPLC-MS/MS; PK-PD model; RAT PLASMA;
D O I
10.3390/molecules28186437
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lonicerae japonicae Flos (LJF) possesses a good anti-respiratory syncytial virus (RSV) effect. However, the material basis of LJF in treating RSV is still unclear. In this study, a sensitive and accurate quantitative method based on UHPLC-QQQ MS was established and validated for the simultaneous determination of the 15 ingredients from LJF in RSV-infected mice plasma. Multiple reaction monitoring was performed for quantification of the standards and of the internal standard in plasma. All the calibration curves show good linear regression within the linear range (r(2) > 0.9918). The method validation results, including specificity, linearity, accuracy, precision, extraction recovery, matrix effect, and stability of 15 ingredients, are all within the current acceptance criteria. This established method was successfully applied to the pharmacokinetic study of 15 compounds from LJF. Furthermore, the repair rate of lung index and the improvement rate of IFN-gamma and IL-6 improved after administration of the LJF, indicating that LJF possessed a positive effect on the treatment of RSV infection. Finally, by combining Spearman and Grey relation analysis, isochlorogenic acid B, isochlorogenic acid C, secoxyloganin, chlorogenic acid, and loganic acid are speculated to be the main effective ingredients of LJF in treating RSV. This study lays the foundation for attempts to reveal the mechanisms of the anti-RSV effect of LJF.
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页数:19
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