Survey of the Endophytic Bacteria Inhabiting Wild Daucus Seed Using 16S rRNA Gene Amplicon Sequencing

被引:3
|
作者
Kumar, Sandeep [1 ]
Johnson, Linda J. J. [1 ]
Teasdale, Suliana [1 ,2 ]
Morozova, Yulia [1 ]
de Bonth, Anouck C. M. [1 ]
Jauregui, Ruy [1 ,3 ]
Hannaford, Rina [1 ]
Card, Stuart D. D. [1 ]
机构
[1] AgResearch, Grasslands Res Ctr, Palmerston North, New Zealand
[2] Dept Conservat, Wellington, New Zealand
[3] Minist Primary Ind, Upper Hutt, New Zealand
来源
MICROBIOLOGY RESOURCE ANNOUNCEMENTS | 2023年 / 12卷 / 06期
关键词
D O I
10.1128/mra.00140-23
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We report a preliminary survey of the endophytic bacterial microbiota of seed from wild carrot (Daucus carota) using 16S rRNA gene amplicon sequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most abundant phyla detected, while Bacillus, Massilia, Paenibacillus, Pantoea, Pseudomonas, Rhizobium, Sphingomonas, and Xanthomonas were the most abundant genera. We report a preliminary survey of the endophytic bacterial microbiota of seed from wild carrot (Daucus carota) using 16S rRNA gene amplicon sequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most abundant phyla detected, while Bacillus, Massilia, Paenibacillus, Pantoea, Pseudomonas, Rhizobium, Sphingomonas, and Xanthomonas were the most abundant genera.
引用
收藏
页数:3
相关论文
共 50 条
  • [1] 16S rRNA Amplicon Sequencing for Epidemiological Surveys of Bacteria in Wildlife
    Galan, Maxime
    Razzauti, Maria
    Bard, Emilie
    Bernard, Maria
    Brouat, Carine
    Charbonnel, Nathalie
    Dehne-Garcia, Alexandre
    Loiseau, Anne
    Tatard, Caroline
    Tamisier, Lucie
    Vayssier-Taussat, Muriel
    Vignes, Helene
    Cosson, Jean-Francois
    MSYSTEMS, 2016, 1 (04)
  • [2] Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing
    Fujimoto, Naoshi
    Mizuno, Keigo
    Yokoyama, Tomoki
    Ohnishi, Akihiro
    Suzuki, Masaharu
    Watanabe, Satoru
    Komatsu, Kenji
    Sakata, Yoichi
    Kishida, Naohiro
    Akiba, Michihiro
    Matsukura, Satoko
    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2015, 61 (05): : 171 - 176
  • [3] Computational analysis of microbial community using amplicon sequencing of 16S rRNA gene
    Singh, N.
    Singh, M. P.
    RESEARCH JOURNAL OF BIOTECHNOLOGY, 2022, 17 (05): : 143 - 150
  • [4] Detection of associated bacteria in aspiration pneumonia and lung abscesses using partial 16S rRNA gene amplicon sequencing
    Aoki, Kotaro
    Ishii, Yoshikazu
    Tateda, Kazuhiro
    ANAEROBE, 2021, 69
  • [5] Detection of pathogenic bacteria in the blood from sepsis patients using 16S rRNA gene amplicon sequencing analysis
    Watanabe, Nobuo
    Kryukov, Kirill
    Nakagawa, So
    Takeuchi, Junko S.
    Takeshita, Meiko
    Kirimura, Yukiko
    Mitsuhashi, Satomi
    Ishihara, Toru
    Aoki, Hiromichi
    Inokuchi, Sadaki
    Imanishi, Tadashi
    Inoue, Shigeaki
    PLOS ONE, 2018, 13 (08):
  • [6] Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
    Marotz, Clarisse
    Sharma, Anukriti
    Humphrey, Greg
    Gottel, Neil
    Daum, Christopher
    Gilbert, Jack A.
    Eloe-Fadrosh, Emiley
    Knight, Rob
    BIOTECHNIQUES, 2019, 67 (01) : 29 - 32
  • [7] Optimization of a method for diversity analysis of anammox bacteria using high-throughput sequencing of 16S rRNA gene amplicon
    Wen, Xiaomei
    Hong, Yiguo
    Wu, Jiapeng
    Wang, Yu
    JOURNAL OF MICROBIOLOGICAL METHODS, 2020, 178
  • [8] 16S rRNA amplicon sequencing of organic and conventional chickens
    Punchihewage-Don, Anuradha J.
    Hasan, Nur A.
    Parveen, Salina
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2025, 14 (02)
  • [9] 16S rRNA Gene Amplicon Sequencing Data for Pteris vittata Rhizosphere Soils
    Mu'azu, Aminu Salisu
    Haris, Hazzeman
    Zarkasi, Kamarul Zaman
    Lau, Nyok-Sean
    Ghazali, Amir Hamzah
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2023, 12 (03):
  • [10] Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
    Coelho, Mariza M.
    Ferreira-Nozawa, Monica S.
    Nozawa, Sergio R.
    Santos, Andre L. W.
    GENETICS AND MOLECULAR BIOLOGY, 2011, 34 (04) : 676 - U257