The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions

被引：3

作者：

Subramaniyan, Bharathiraja ^{[1
]}

Gurung, Sunam ^{[2
]}

Bodas, Manish ^{[1
]}

Moore, Andrew R. ^{[1
]}

Larabee, Jason L. ^{[3
]}

Reuter, Darlene ^{[4
]}

Georgescu, Constantin ^{[5
]}

Wren, Jonathan D. ^{[5
]}

Myers, Dean A. ^{[2
]}

Papin, James F. ^{[4
,6
]}

Walters, Matthew S. ^{[1
]}

机构：

[1] Univ Oklahoma, Hlth Sci Ctr, Dept Med, Sect Pulm Crit Care & Sleep Med, Oklahoma City, OK 73104 USA

[2] Univ Oklahoma, Hlth Sci Ctr, Dept Obstet & Gynecol, Oklahoma City, OK 73104 USA

[3] Univ Oklahoma, Hlth Sci Ctr, Dept Microbiol & Immunol, Oklahoma City, OK 73104 USA

[4] Univ Oklahoma, Hlth Sci Ctr, Div Comparat Med, Oklahoma City, OK 73104 USA

[5] Oklahoma Med Res Fdn, Genes & Human Dis Res Program, Oklahoma City, OK 73104 USA

[6] Univ Oklahoma, Hlth Sci Ctr, Dept Pathol, Oklahoma City, OK 73104 USA

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 04期

关键词：

COVID-19; SARS-CoV-2; non-human primate; baboon; air-liquid interface; airway epithelium; immune response; innate immunity; inflammation; INFANT BABOONS; LUNG; MODELS; REPAIR; REGENERATION; PATHOGENESIS; PLASTICITY; COVID-19; DISEASE;

D O I：

10.3390/v15040862

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The mucociliary airway epithelium lines the human airways and is the primary site of host-environmental interactions in the lung. Following virus infection, airway epithelial cells initiate an innate immune response to suppress virus replication. Therefore, defining the virus-host interactions of the mucociliary airway epithelium is critical for understanding the mechanisms that regulate virus infection, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Non-human primates (NHP) are closely related to humans and provide a model to study human disease. However, ethical considerations and high costs can restrict the use of in vivo NHP models. Therefore, there is a need to develop in vitro NHP models of human respiratory virus infection that would allow for rapidly characterizing virus tropism and the suitability of specific NHP species to model human infection. Using the olive baboon (Papio anubis), we have developed methodologies for the isolation, in vitro expansion, cryopreservation, and mucociliary differentiation of primary fetal baboon tracheal epithelial cells (FBTECs). Furthermore, we demonstrate that in vitro differentiated FBTECs are permissive to SARS-CoV-2 infection and produce a potent host innate-immune response. In summary, we have developed an in vitro NHP model that provides a platform for the study of SARS-CoV-2 infection and other human respiratory viruses.

引用

页数：21