Three-phase extraction of polysaccharide from Stropharia rugosoannulata: Process optimization, structural characterization and bioactivities

被引:22
作者
Li, Xinxin [1 ,2 ,3 ]
Zhang, Zhiqiang [2 ]
Wang, Li [1 ]
Zhao, Haoqiang [1 ]
Jia, Yahui [1 ]
Ma, Xia [3 ]
Li, Jinzhan [4 ]
Wang, Yi [5 ]
Ma, Bingji [1 ]
机构
[1] Henan Agr Univ, Dept Tradit Chinese Med, Zhengzhou, Peoples R China
[2] Henan Univ Chinese Med, Sch Pharm, Zhengzhou, Peoples R China
[3] Henan Univ Anim Husb & Econ, Coll Anim medcine, Zhengzhou, Peoples R China
[4] Henan Jinlong Mushroom Ind Co LTD, Shangqiu, Peoples R China
[5] GeneGenieDx Corp, Business Dev, San Jose, CA USA
基金
中国国家自然科学基金;
关键词
Stropharia rugosoannulata; polysaccharide; three-phase extraction; structure; bioactivities; SIGNALING PATHWAYS; IN-VITRO; ANTIOXIDANT; PURIFICATION; AUTOPHAGY; MUSHROOM; SOLVENT; CELLS; SEED;
D O I
10.3389/fimmu.2022.994706
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The isolation of Stropharia rugosoannulata polysaccharide (SRP) by three-phase extraction was optimized, and its structure and biological activities were identified. The optimal extraction conditions were: mass fraction of ammonium sulfate, 20%; volume ratio of sample solution to t-butanol, 1:1.5; extraction temperature, 35 degrees C. Under these conditions, the yield of SRP was 6.85% +/- 0.13%. SRP was found to be composed of glucose (35.79%), galactose (26.80%), glucuronic acid (9.92%), fructose (8.65%), xylose (7.92%), fucose (4.19%), arabinose (3.46%) and rhamnose (3.26%), with the molecular weight of 27.52 kDa. The results of DPPH, hydroxyl, ABTS(+) radical scavenging and reducing power tests showed that SRP had good antioxidant capacities. SRP had no cytotoxic effect on RAW264.7 macrophages at the concentrations of 25-200 mu g/mL, and could significantly promote phagocytosis activity and cell migration according to CCK-8 assay, phagocytosis assay and cell scratch experiment. SRP can significantly stimulate the transcript expression levels of TNF-alpha, IL-1 beta and IL-6, as determined by RT-PCR and Western blot assays. SRP activated the TLR4/NF-kappa B signaling pathway, and autophagy also occurred. These results suggest that SRP is a safe antioxidant and immunomodulator, and that it can be used in the development of functional foods and/or pharmaceuticals.
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页数:14
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