Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub

被引:3
|
作者
Zhao, Shi-Wei [1 ]
Guo, Jing-Fang [1 ]
Kong, Lei [1 ]
Nie, Shuai [1 ]
Yan, Xue-Mei [1 ]
Shi, Tian-Le [1 ]
Tian, Xue-Chan [1 ]
Ma, Hai-Yao [1 ]
Bao, Yu-Tao [1 ]
Li, Zhi-Chao [1 ]
Chen, Zhao-Yang [1 ]
Zhang, Ren-Gang [2 ]
Ma, Yong-Peng [2 ]
El-Kassaby, Yousry A. [3 ]
Porth, Ilga [4 ]
Zhao, Wei [5 ]
Mao, Jian-Feng [1 ,6 ]
机构
[1] Beijing Forestry Univ, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Coll Biol Sci & Technol,Minist Educ,Key Lab Genet, Beijing 100083, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Integrat Conservat Plant Species ex, Kunming 650201, Peoples R China
[3] Univ British Columbia, Fac Forestry, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada
[4] Univ Laval, Fac Foresterie Geog & Geomat, Dept Sci Bois & Foret, Quebec City, PQ 106, Canada
[5] Umea Univ, Umea Plant Sci Ctr, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden
[6] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, SE-90187 Umea, Sweden
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
NUCLEOTIDE-SEQUENCES; ESSENTIAL OIL; ROOT-NODULES; ANNOTATION; TRANSCRIPTOME; PATTERNS; PROVIDES; PROGRAM; SYSTEM; GROWTH;
D O I
10.1038/s41597-023-02171-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.
引用
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页数:11
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