The toxicity assessment of neorudin in cynomolgus monkeys

被引:0
作者
Liu, Yu-bin [1 ]
Renl, Bo-yuan [1 ]
Zhou, Xing-chen [1 ]
Zhang, Lin [1 ]
Liu, Yun [1 ]
Zheng, Can [1 ]
Weil, Jin-feng [2 ]
Wu, Chu-tse [1 ]
Jin, Ji-de [1 ]
机构
[1] Beijing Inst Radiat Med, Beijing 100850, Peoples R China
[2] Beijing Union Jianhao Med Technol Dev Co Ltd, Beijing 100176, Peoples R China
关键词
Neorudin; Anticoagulant; Toxicity assessment; Cynomolgus monkeys; MOLECULAR-WEIGHT HEPARIN; HIRUDIN; INTERVAL; GENDER;
D O I
暂无
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
In this study, the toxicity effects on circulatory system and respiratory system, and the acute toxicity test of recombinant neorudin (EPR-hirudin, EH) in cynomolgus monkeys were evaluated to provide reference information for clinical studies. Eighteen cynomolgus monkeys were randomly divided into three groups for single intravenous administration of 3, 30 mg/kg EH and normal saline, respectively. The changes of respiratory frequency, respiratory intensity, blood pressure and electrocardiogram before and after administration were recorded. In acute toxicity test, six cynomolgus monkeys were intravenous-ly received EH at a single dose of 171, 257, 385, 578, 867 and 1300 mg/kg respectively. The vital signs, hematology, serum biochemistry, coagulation indexes and electrocardiogram indexes of the animals were determined before administration and on the 7th and 14th day after administration. As the results showed that there were no significant abnormal changes in respiratory frequency, respiratory intensity, blood pres-sure or electrocardiogram in cynomolgus monkeys after receiving EH at 3 mg/kg and 30 mg/kg, and there was no statistical difference between the treated groups and normal saline group. In the acute toxic-ity test, no significant abnormalities were observed in vital signs, hematology, serum biochemistry, coag-ulation indexes and electrocardiogram indexes of six cynomolgus monkeys at day 7 and 14 after EH administration. Furthermore, autopsies of all cynomolgus monkeys showed no abnormalities. The results of toxicokinetics showed that AUClast of the drug increased in proportion to the EH dose in the range of 171-578 mg/kg, and increased in over proportion to the EH dose in the range of 578-1300 mg/kg. The variation of Cmax was basically consistent with AUClast. In a sum, A single intravenous injection of 3 and 30 mg/kg of EH did not affect the circulatory system and respiratory system in cynomolgus mon-keys and the maximum tolerated dose of EH in cynomolgus monkey is over 1300 mg/kg (equivalent to 619-1300 times of the proposed clinical equivalent dose).
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页码:179 / 189
页数:11
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