Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system

被引：0

作者：

Ding, Tongbin ^{[1
]}

Gao, Zhe ^{[1
]}

Liu, Hang ^{[1
]}

Li, Yuan ^{[1
]}

Liu, Shichao ^{[1
]}

Jian, Liguo ^{[1
,2
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 2, Dept Cardiol, Zhengzhou 450014, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 2, Dept Cardiol, 2 Jingba Rd, Zhengzhou 450014, Henan, Peoples R China

来源：

STEM CELL RESEARCH | 2023年 / 67卷

关键词：

D O I：

10.1016/j.scr.2023.103029

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Protein phosphatase 2A (PPP2CA) is one of the four main Ser/Thr phosphatase enzymes, which involved in the negative control of cell growth and division. PPP2CA is the main protein phosphatase in the heart, which regulates Ca (2+) through a series of ion channels and transporters. In this study, we generated a PPP2CA homozygous knockout human embryonic stem cell line WAe009-A-25 based on the transient expression CRISPR/Cas9 system to investigate functional effect of PP1 deficiency. This cell line has multidirectional differentiation potential, normal karyotypic and trilineage differentiation potential in vivo.

引用

页数：5