Isochrysis sp. cultivation in pilot-scale to concurrently produce sustainable triacylglycerols for human milk fat substitutes and fucoxanthin

被引：2

作者：

Li, Daogui ^{[1
]}

Chen, Na ^{[1
]}

Zhou, Zhihua ^{[1
]}

Zhong, Cairong ^{[1
]}

Fang, Jingping ^{[1
]}

Huang, Luqiang ^{[1
]}

Sun, Han ^{[2
]}

Chi, Chengdeng ^{[1
]}

Zhou, Youcai ^{[1
]}

He, Yongjin ^{[1
,3
]}

机构：

[1] Fujian Normal Univ, Coll Life Sci, 1 Keji Rd, Fuzhou 350117, Minhou, Peoples R China

[2] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

[3] Fujian Normal Univ, Engn Res Ctr Ind Microbiol, Minist Educ, 1 Keji Rd, Fuzhou 350117, Minhou, Peoples R China

来源：

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2023年 / 69卷

基金：

中国国家自然科学基金;

关键词：

Isochrysis sp; Human milk fat substitutes; Triacylglycerols; Fucoxanthin; Cultivation; SEMICONTINUOUS PROCESS; ALGAL BIOMASS; MICROALGAE; CULTURES; STRATEGY; STRAINS; BATCH;

D O I：

10.1016/j.algal.2022.102937

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Isochcrysis strains as versatile cell factories can produce varieties of high-valued bioproducts for food, nutra-ceuticals and pharmaceuticals. This work aimed to evaluate the ability of Isochrysis sp. to produce high-valued triacylglycerols (TAGs) for human milk fat substitutes (HMFs) and fucoxanthin by batch, fed-batch and semi -continuous cultivation modes in 50-L column photobioreactors. It was found that, Isochrysis cells by three cultivation modes had individual growth characteristics, nutrients consumption, and biochemical composition. In all cultivation modes, microalga cultivated by the semi-continuous cultivation at 10 % renewable rate achieves the highest TAGs and fucoxanthin productivities with 8.2 and 0.5 mg/(L.d), respectively. Additionally, the ob-tained results showed that the microalgal growth phase and the levels of nitrogen and phosphorus had distinct impacts on the deducting G value for microalgal TAGs produced by three cultivation modes. Based on the fatty acid composition and positional distribution, Isochrysis TAGs by the semi-continuous process at 10 % renewable rate had 73.0 deducting G value that were close to the ones by the batch cultivation (13th day) and fed-batch cultivation. Taken together, Isochrysis sp. was the promising marine microalga to concurrently produce sus-tainable high-valued TAGs for HMFs and fucoxanthin by semi-continuous cultivation in pilot-scale photo-bioreactors. This work provides a new marine microalgal platform to exploit Isochrysis-derived TAGs as human milk fat subsitutes for infant formulas and fucoxanthin for nutraceuticals and pharmaceuticals.

引用

页数：10