Transcription induces context-dependent remodeling of chromatin architecture during differentiation

被引:6
作者
Chahar, Sanjay [1 ]
Ben Zouari, Yousra [1 ]
Salari, Hossein [2 ]
Kobi, Dominique [1 ]
Maroquenne, Manon [1 ]
Erb, Cathie [1 ]
Molitor, Anne M. [1 ]
Mossler, Audrey [1 ]
Karasu, Nezih [1 ]
Jost, Daniel [2 ]
Sexton, Tom [1 ]
机构
[1] Univ Strasbourg, CNRS, INSERM, IGBMC,UMR 7104,UMR S 1258, Illkirch Graffenstaden, France
[2] Univ Claude Bernard Lyon 1, Ecole Normale Super Lyon, Lab Biol & Modelisat Cellule, CNRS,INSERM,UMR5239, Lyon, France
基金
欧洲研究理事会;
关键词
CHROMOSOME DOMAINS; MAMMALIAN GENOMES; X-CHROMOSOME; CTCF; PRINCIPLES; COHESIN; ORGANIZATION; REVEALS; INSULATION; TOPOLOGY;
D O I
10.1371/journal.pbio.3002424
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Metazoan chromosomes are organized into discrete spatial domains (TADs), believed to contribute to the regulation of transcriptional programs. Despite extensive correlation between domain organization and gene activity, a direct mechanistic link is unclear, with perturbation studies often showing little effect. To follow chromatin architecture changes during development, we used Capture Hi-C to interrogate the domains around key differentially expressed genes during mouse thymocyte maturation, uncovering specific remodeling events. Notably, one TAD boundary was broadened to accommodate RNA polymerase elongation past the border, and subdomains were formed around some activated genes without changes in CTCF binding. The ectopic induction of some genes was sufficient to recapitulate domain formation in embryonic stem cells, providing strong evidence that transcription can directly remodel chromatin structure. These results suggest that transcriptional processes drive complex chromosome folding patterns that can be important in certain genomic contexts. Links between genome organization and transcriptional control have been controversial and unclear. Although most TADs (topologically associated domains) are stable through development, this study shows by ectopic induction in ESCs that transcription can directly remodel TADs, with a context-dependent sensitivity.
引用
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页数:29
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