Galectin-8 Promotes Gastric Cancer Cell Proliferation by Activating the Ras Signaling Pathway

被引:0
|
作者
Zhu, Xiao-dong [1 ]
Zhang, Qi [1 ]
Wang, Yang [1 ]
Jiang, Ming-rui [1 ]
Ali, Muhammad [1 ]
Sun, Qian-nan [2 ]
Wang, Dao-rong [2 ]
机构
[1] Yangzhou Univ, Clin Med Coll, Yangzhou 225009, Jiangsu, Peoples R China
[2] Northern Jiangsu Peoples Hosp, Dept Gen Surg, Yangzhou 225001, Jiangsu, Peoples R China
来源
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS | 2023年 / 37卷 / 03期
基金
中国国家自然科学基金;
关键词
gastric cancer (GC); galectin-8; cell proliferation; Ras signaling pathway; LECTIN;
D O I
10.23812/j.biol.regul.homeost.agents.20233703.129
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Gastric cancer (GC) is one of the most common malignant tumors worldwide, but due to its complex pathogenesis, current treatments are still very limited. Studies have shown that galectin-8 expression is significantly increased in GC and is associated with poor prognosis in patients, but the mechanism of galectin-8 in GC remains unclear. Therefore, this study aimed to investigate the expression, prognostic value, and molecular mechanism of galectin-8 in GC.Methods: UALCAN was used to analyze galectin-8 expression levels in GC and their correlation with clinical stage, histological subtype, and lymph node metastasis. Western blotting and immunohistochemical staining were used to detect the expression levels of galectin-8 in GC tissues. siRNA was transfected using lentivirus to knock down galectin-8. Galectin-8 expression levels and transfection efficacy in GC cells were detected using Western blot. The Cell Counting Kit-8 (CCK-8) assay and colony formation assay were used to detect the level of cell proliferation. Western blotting was used to detect the rat sarcoma viral oncogene homolog (Ras) signaling pathway-related protein expression levels. Immunofluorescence staining was performed for co-localization analysis of galectin-8 and Kirsten Ras (K-Ras) proteins.Results: The expression levels of galectin-8 were significantly increased in GC tissues and cells, and that was positively correlated with clinical stage, histological subtype and lymph node metastasis, and negatively correlated with overall survival. Knockdown of galectin-8 significantly inhibited Human Gastric Adenocarcinoma (AGS) cell proliferation and decreased RAF proto-oncogene serine/threonine-protein kinase (Raf), mitogen-activated protein kinase (Mek), and K-Ras protein expression levels. Immunoflu-orescence staining results indicated that galectin-8 and K-Ras protein co-localized in the cytoplasm.Conclusions: Galectin-8 promotes GC proliferation by activating the Ras signaling pathway and is a potential therapeutic target and prognostic biomarker for GC.
引用
收藏
页码:1285 / 1291
页数:7
相关论文
共 50 条
  • [21] Galectin-8 promotes migration and proliferation and prevents apoptosis in U87 glioblastoma cells
    Claudia Metz
    Remziye Döger
    Elizabeth Riquelme
    Priscilla Cortés
    Christopher Holmes
    Ronan Shaughnessy
    Claudia Oyanadel
    Catalina Grabowski
    Alfonso González
    Andrea Soza
    Biological Research, 49
  • [22] LINC00665 promotes cell proliferation, invasion, and metastasis by activating the TGF-β pathway in gastric cancer
    Zhang, Xiaohu
    Wu, Jixiang
    PATHOLOGY RESEARCH AND PRACTICE, 2021, 224
  • [23] EZR promotes pancreatic cancer proliferation and metastasis by activating FAK/AKT signaling pathway
    Jian Xu
    Wei Zhang
    Cancer Cell International, 21
  • [24] ARHGEF39 promotes gastric cancer cell proliferation and migration via Akt signaling pathway
    Haixiao Wang
    Miaomiao Li
    Xiaobao Tao
    Yan Qian
    Linfang Chen
    Guoquan Tao
    Molecular and Cellular Biochemistry, 2018, 440 : 33 - 42
  • [25] ARMC8α promotes proliferation and invasion of non-small cell lung cancer cells by activating the canonical Wnt signaling pathway
    Xie, Chengyao
    Jiang, Guiyang
    Fan, Chuifeng
    Zhang, Xiupeng
    Zhang, Yong
    Miao, Yuan
    Lin, Xuyong
    Wu, Junhua
    Wang, Liang
    Liu, Yang
    Yu, Juanhan
    Yang, Lianhe
    Zhang, Di
    Xu, Ke
    Wang, Enhua
    TUMOR BIOLOGY, 2014, 35 (09) : 8903 - 8911
  • [26] ARHGEF39 promotes gastric cancer cell proliferation and migration via Akt signaling pathway
    Wang, Haixiao
    Li, Miaomiao
    Tao, Xiaobao
    Qian, Yan
    Chen, Linfang
    Tao, Guoquan
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2018, 440 (1-2) : 33 - 42
  • [27] EZR promotes pancreatic cancer proliferation and metastasis by activating FAK/AKT signaling pathway
    Xu, Jian
    Zhang, Wei
    CANCER CELL INTERNATIONAL, 2021, 21 (01)
  • [28] Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling
    Diskin, Shiri
    Chen, Wei-Sheng
    Cao, Zhiyi
    Gyawali, Smita
    Gong, Haiyan
    Soza, Andrea
    Gonzalez, Alfonso
    Panjwani, Noorjahan
    PLOS ONE, 2012, 7 (09):
  • [29] Galectin-8 alters immune microenvironment and promotes tumor progression
    Wang, Yiting
    Sun, Yufan
    Li, Hai
    Xu, Jie
    AMERICAN JOURNAL OF CANCER RESEARCH, 2023, 13 (06): : 2517 - +
  • [30] CLDN8 promotes colorectal cancer cell proliferation, migration, and invasion by activating MAPK/ERK signaling
    Cheng, Bo
    Rong, Aimei
    Zhou, Quanbo
    Li, Wenlu
    CANCER MANAGEMENT AND RESEARCH, 2019, 11 : 3741 - 3751