Rps6ka2 enhances iMSC chondrogenic differentiation to attenuate knee osteoarthritis through articular cartilage regeneration in mice

被引：1

作者：

Zhang, Juan ^{[1
,2
]}

Liao, Jin-Qi ^{[3
]}

Wen, Li-Ru ^{[1
]}

Padhiar, Arshad-Ahmed ^{[1
]}

Li, Zhu ^{[1
]}

He, Zhong-Yuan ^{[4
]}

Wu, Hua-Chuan ^{[4
]}

Li, Jian-Feng ^{[4
]}

Zhang, Shuai ^{[5
,6
]}

Zhou, Yan ^{[1
,3
]}

Pan, Xiao-Hua ^{[7
]}

Yang, Jian-Hua ^{[8
]}

Zhou, Guang-Qian ^{[1
]}

机构：

[1] Shenzhen Univ, Hlth Sci Ctr, Dept Med Cell Biol & Genet,Shenzhen Engn Lab Regen, Guangdong Key Lab Genom Stabil & Dis Prevent,Shenz, Shenzhen 518107, Peoples R China

[2] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Endocrinol, Hengyang 421001, Hunan, Peoples R China

[3] Lungene Biotech Ltd, Shenzhen 518107, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 7, Dept Orthopaed Surg, Innovat Platform Regenerat & Repair Spinal Cord &, Shenzhen 518107, Peoples R China

[5] Southern Univ Sci & Technol, Brain Res Ctr, Shenzhen 518107, Peoples R China

[6] Southern Univ Sci & Technol, Dept Biol, Shenzhen 518107, Peoples R China

[7] Southern Med Univ, Shenzhen Univ, Affiliated Hosp 2,Dept Orthopaed,Peoples Hosp Shen, Clin Med Coll,Guangdong Med Univ,Sch Clin Med 2, Shenzhen 518107, Peoples R China

[8] Chinese Univ Hong Kong, Affiliated Hosp 2, Shenzhen & Longgang Dist Peoples Hosp Shenzhen, Shenzhen 518107, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2023年 / 663卷

基金：

中国国家自然科学基金;

关键词：

Knee osteoarthritis; Articular cartilage regeneration; Rps6ka2; iMSC; Chondrogenic differentiation; ROLES;

D O I：

10.1016/j.bbrc.2023.04.049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Articular cartilage (AC) is most susceptible to degeneration in knee osteoarthritis (OA); however, the existing treatments for OA do not target the core link of the pathogenesis-"decreased tissue cell function activity and extracellular matrix (ECM) metabolic disorders" for effective intervention. iMSC hold lower heterogeneity and great promise in biological research and clinical applications. Rps6ka2 may play an important role in the iMSC to treat OA. In this study, the CRISPR/Cas9 gene editing Rps6ka2-/- iMSC were obtained. Effect of Rps6ka2 on iMSC proliferation and chondrogenic differentiation was evaluated in vitro. An OA model was constructed in mice by surgical destabilization of medial meniscus (DMM). The Rps6ka2-/- iMSC and iMSC were injected into the articular cavity twice-weekly for 8 weeks. In vitro experiments showed that Rps6ka2 could promote iMSC proliferation and chondrogenic differentiation. In vivo results further confirmed that Rps6ka2 could improve iMSC viability to promote ECM production to attenuate OA in mice. (c) 2023 Elsevier Inc. All rights reserved.

引用

页码：61 / 70

页数：10

共 1 条

[1] Senescent chondrogenic progenitor cells derived from articular cartilage of knee osteoarthritis patients contributes to senescence-associated secretory phenotype via release of IL-6 and IL-8
Jacob, Justin
Aggarwal, Anjali
Aggarwal, Aditya
Bhattacharyya, Shalmoli
Kumar, Vishal
Sharma, Vinit
Sahni, Daisy
ACTA HISTOCHEMICA, 2022, 124 (03)

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