Fast cryo-electron tomography data acquisition to determine molecular structures in situ

被引：0

作者：

Chlanda, Petr ^{[1
]}

机构：

[1] Univ Hosp Heidelberg, Heidelberg, Germany

来源：

NATURE METHODS | 2023年 / 20卷 / 01期

关键词：

CRYO-EM;

D O I：

10.1038/s41592-022-01691-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To accelerate data acquisition for in situ cryo-electron tomography, we created a method that takes into consideration sample geometry for the robust prediction of sample movement while the microscope stage is tilted. This approach enabled the parallel collection of tens to hundreds of tilt series.

引用

页码：42 / 43

页数：2

共 5 条

[1] High resolution single particle cryo-electron microscopy using beam-image shift [J].