Protocol for producing an adeno-associated virus vector by controlling capsid expression timing

被引:0
|
作者
Ohba, Kenji [1 ]
Mizukami, Hiroaki [1 ]
机构
[1] Jichi Med Univ, Ctr Mol Med, Div Genet Therapeut, Shimotsuke, Tochigi 3290498, Japan
来源
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金
日本学术振兴会;
关键词
GENE-THERAPY; AAV; EFFICIENT;
D O I
10.1016/j.xpro.2023.102542
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Conventional adeno-associated virus (AAV) production systems generate vast numbers of empty capsids, which should be eliminated before clinical use. Here, we present a protocol for efficient AAV vector production. We describe steps for separating replicase and capsid genes from the plasmid and controlling capsid expression until sufficient AAV vector genome replication is achieved. This protocol can produce AAV vectors in various serotypes. For complete details on the use and execution of this protocol, please refer to Ohba et al.1
引用
收藏
页数:17
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