A rapid PCR-based method to determine the Neurospora crassa mating type

被引：0
|
作者：
Schaefer, Anke [1 ,2 ]
Kempken, Frank [1 ,2 ,3 ,4 ]
机构：
[1] Bot Inst, Abt Bot Genet & Mol Biol, Kiel, Germany
[2] Bot Garten, Abt Bot Genet & Mol Biol, Kiel, Germany
[3] Christian Albrechts Univ Kiel, Bot Inst, Abt Bot Genet & Mol Biol, Olshausenstr 40, D-24098 Kiel, Germany
[4] Christian Albrechts Univ Kiel, Bot Garten, Olshausenstr 40, D-24098 Kiel, Germany
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2024年 / 64卷 / 02期
关键词：
mating type; Neurospora crassa; PCR-based mating type identification;
D O I：
10.1002/jobm.202300495
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
So far mating type determination in Neurospora crassa requires test crosses with strains of known mating type. We present a simple, quick, and reliable polymerase chain reaction-based method for mating type determination in N. crassa.
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页数：4
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