Effects of hydroxyapatite-coated porous titanium scaffolds functionalized by exosomes on the regeneration and repair of irregular bone

被引:6
作者
Shao, Hanyu [1 ]
Zhang, Qiyue [2 ]
Sun, Mingman [1 ]
Wu, Ming [1 ]
Sun, Xu [1 ]
Wang, Qiang [2 ]
Tong, Shuang [1 ]
机构
[1] China Med Univ, Hosp 1, Dept Plast Surg, Shenyang, Peoples R China
[2] China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang, Peoples R China
关键词
adipose-derived stem cells; exosomes; bone tissue engineering; porous titanium alloy scaffold; hydroxyapatite; MESENCHYMAL STEM-CELLS; EXTRACELLULAR VESICLES; CALCIUM-PHOSPHATE; IN-VITRO; CARTILAGE; IMPLANTS; DESIGN; ALLOY;
D O I
10.3389/fbioe.2023.1283811
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
As a traditional bone implant material, titanium (Ti) and its alloys have the disadvantages of lack of biological activity and susceptibility to stress shielding effect. Adipose stem cells (ADSCs) and exosomes were combined with the scaffold material in the current work to effectively create a hydroxyapatite (HA) coated porous titanium alloy scaffold that can load ADSCs and release exosomes over time. The composite made up for the drawbacks of traditional titanium alloy materials with higher mechanical characteristics and a quicker rate of osseointegration. Exosomes (Exos) are capable of promoting the development of ADSCs in porous titanium alloy scaffolds with HA coating, based on experimental findings from in vitro and in vivo research. Additionally, compared to pure Ti implants, the HA scaffolds loaded with adipose stem cell exosomes demonstrated improved bone regeneration capability and bone integration ability. It offers a theoretical foundation for the combined use of stem cell treatment and bone tissue engineering, as well as a design concept for the creation and use of novel clinical bone defect repair materials.
引用
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页数:12
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