Improved cryptic plasmids in probiotic Escherichia coli Nissle 1917 for antibiotic-free pathway engineering
被引:3
作者:
Dong, Miao-Miao
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机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Dong, Miao-Miao
[1
]
Song, Lu
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机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Song, Lu
[1
]
Xu, Jia-Qi
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h-index: 0
机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Xu, Jia-Qi
[1
]
Zhu, Lin
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h-index: 0
机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Zhu, Lin
[1
]
Xiong, Liang-Bin
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h-index: 0
机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Shanghai Univ Med & Hlth Sci, Sch Pharm, Shanghai 201318, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Xiong, Liang-Bin
[1
,2
]
Wei, Dong-Zhi
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机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Wei, Dong-Zhi
[1
]
Wang, Feng-Qing
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h-index: 0
机构:
East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R ChinaEast China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Wang, Feng-Qing
[1
]
机构:
[1] East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
[2] Shanghai Univ Med & Hlth Sci, Sch Pharm, Shanghai 201318, Peoples R China
The engineered probiotic Escherichia coli Nissle 1917 (EcN) is expected to be employed in the diagnosis and treatment of various diseases. However, the introduced plasmids typically require antibiotics to maintain genetic stability, and the cryptic plasmids in EcN are usually eliminated to avoid plasmid incompatibility which may change the inherent probiotic characteristics. Here, we provided a simple design to minimize the genetic change of probiotics by eliminating native plasmids and reintroducing the recombinants carrying functional genes. Specific insertion sites in the vectors showed significant differences in the expression of fluorescence proteins. Selected integration sites were applied in the de novo synthesis of salicylic acid, leading to a titer of 142.0 +/- 6.0 mg/L in a shake flask with good production stability. Additionally, the design successfully realized the biosynthesis of ergothioneine (45 mg/L) by one-step construction. This work expands the application scope of native cryptic plasmids to the easy construction of functional pathways.
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Chen, Haojie
Lei, Pengyu
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Lei, Pengyu
Ji, Hao
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Ji, Hao
Yang, Qinsi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chinese Acad Sci, Wenzhou Inst, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Yang, Qinsi
Peng, Bo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chinese Acad Sci, Wenzhou Inst, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Peng, Bo
Ma, Jiahui
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Ma, Jiahui
Fang, Yimeng
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Fang, Yimeng
Qu, Linkai
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R China
Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Qu, Linkai
Li, Hua
论文数: 0引用数: 0
h-index: 0
机构:
Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Li, Hua
Wu, Wei
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h-index: 0
机构:
Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wu, Wei
Jin, Libo
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R China
Wenzhou City & WenZhouOuTai Med Lab Co Ltd Joint D, Wenzhou Assoc Sci & Technol, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Jin, Libo
Sun, Da
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R China
Wenzhou City & Kunlong Technol Co Ltd Joint Doctor, Wenzhou Assoc Sci & Technol, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Faith, Jeremiah J.
Guruge, Janaki L.
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h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Guruge, Janaki L.
Charbonneau, Mark
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Charbonneau, Mark
Subramanian, Sathish
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Subramanian, Sathish
Seedorf, Henning
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h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Seedorf, Henning
Goodman, Andrew L.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Goodman, Andrew L.
Clemente, Jose C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Clemente, Jose C.
Knight, Rob
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
Univ Colorado, Biofrontiers Inst, Boulder, CO 80309 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Knight, Rob
Heath, Andrew C.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Psychiat, St Louis, MO 63110 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Heath, Andrew C.
Leibel, Rudolph L.
论文数: 0引用数: 0
h-index: 0
机构:
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Pediat, New York, NY 10032 USA
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Med, New York, NY 10032 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Leibel, Rudolph L.
Rosenbaum, Michael
论文数: 0引用数: 0
h-index: 0
机构:
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Pediat, New York, NY 10032 USA
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Med, New York, NY 10032 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Rosenbaum, Michael
Gordon, Jeffrey I.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Chen, Haojie
Lei, Pengyu
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Lei, Pengyu
Ji, Hao
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Ji, Hao
Yang, Qinsi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chinese Acad Sci, Wenzhou Inst, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Yang, Qinsi
Peng, Bo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chinese Acad Sci, Wenzhou Inst, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Peng, Bo
Ma, Jiahui
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Ma, Jiahui
Fang, Yimeng
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Fang, Yimeng
Qu, Linkai
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R China
Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Qu, Linkai
Li, Hua
论文数: 0引用数: 0
h-index: 0
机构:
Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Li, Hua
Wu, Wei
论文数: 0引用数: 0
h-index: 0
机构:
Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wu, Wei
Jin, Libo
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R China
Wenzhou City & WenZhouOuTai Med Lab Co Ltd Joint D, Wenzhou Assoc Sci & Technol, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Jin, Libo
Sun, Da
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
Wenzhou Univ, Biomed Collaborat Innovat Ctr Zhejiang Prov, Wenzhou 325035, Peoples R China
Wenzhou City & Kunlong Technol Co Ltd Joint Doctor, Wenzhou Assoc Sci & Technol, Wenzhou 325000, Peoples R ChinaWenzhou Univ, Inst Life Sci, Wenzhou 325035, Peoples R China
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Faith, Jeremiah J.
Guruge, Janaki L.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Guruge, Janaki L.
Charbonneau, Mark
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Charbonneau, Mark
Subramanian, Sathish
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Subramanian, Sathish
Seedorf, Henning
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Seedorf, Henning
Goodman, Andrew L.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Goodman, Andrew L.
Clemente, Jose C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Clemente, Jose C.
Knight, Rob
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
Univ Colorado, Biofrontiers Inst, Boulder, CO 80309 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Knight, Rob
Heath, Andrew C.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Psychiat, St Louis, MO 63110 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Heath, Andrew C.
Leibel, Rudolph L.
论文数: 0引用数: 0
h-index: 0
机构:
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Pediat, New York, NY 10032 USA
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Med, New York, NY 10032 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Leibel, Rudolph L.
Rosenbaum, Michael
论文数: 0引用数: 0
h-index: 0
机构:
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Pediat, New York, NY 10032 USA
Columbia Univ Coll Phys & Surg, Div Mol Genet, Dept Med, New York, NY 10032 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA
Rosenbaum, Michael
Gordon, Jeffrey I.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USAWashington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA