Functional near infrared spectroscopy for brain functional connectivity analysis: A graph theoretic approach

Background: Functional Near-Infrared Spectroscopy is an optical brain monitoring technique which uses NIRS to perform functional neuroimaging. It uses near-infrared light for measuring brain activity and to estimate the cortical hemodynamic activity in the brain due to motor ac-tivity. Functional NIRS measures the changes in oxygen levels in oxygenated and deoxygenated hemoglobin by optical absorption. One of the main challenges in the analysis of fNIRS signals is the signal degradation due to the interference from noise and artifacts from multiple sources.Methods: In this context, this research aims to analyze the connectivity between different regions of the brain using graph theory and hence the geometrical association of brain networks in terms of functional parameters. In this study, the impact of two noise removal processes (CBSI and TDDR), along with two types of correlation fNIRS such as Pearson's Correlation (PC), and Cross Correlation (CC) and various whole-brain network architectures on the reproducibility of graph measurements for individual participants has been carefully examined for different densities ranging from 5% to 50%.The graph measures' repeatability at the individual level was studied using the test-retest variability (TRT) Results: The test-retest variability for global measurements in binary networks was substantially large at low densities, regardless of the noise removal method or the kind of correlation. Very low test -reset values are observed for weighted networks and great reproducibility for measures of the entire graph. When comparing the test-retest values for various methods, the kind of corre-lation, the absolute value of the correlation, and the weight calculation method on the raw correlation value all had significant major effects.Conclusion: Based on a weighted network with the absolute cross correlation functioning as the weight, this study revealed that normalized global graph measurements were reliable. The node definition techniques that were utilized to remove noise were not essential for the normalized graph measures to be reproducible.