Hederagenin inhibits proliferation, angiogenesis and inflammation of fibroblast-like synovial cells in rheumatoid arthritis

被引：0

作者：

Wang, Ping ^{[1
]}

Yang, Junli ^{[1
]}

Zhang, Xiaomeng ^{[1
]}

机构：

[1] Anhui Med Univ, Affiliated Hosp 3, Dept Rheumatol, Hefei 230032, Anhui, Peoples R China

来源：

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2023年 / 22卷 / 10期

关键词：

Rheumatoid arthritis; Hederagenin; Inflammation; Angiogenesis; MAPK pathway;

D O I：

10.4314/tjpr.v22i10.4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Purpose: To determine the effect of Hederagenin (Hed) on rheumatoid arthritis (RA) in a cell model, and to elucidate the mechanism of action of Hed.Methods: MTT, EDU, and Immunoblot assays were used to determine the effects of Hed on the viability of fibroblast-like synovial cells, while the effects of Hed on inflammation were examined by enzyme -linked immunosorbent assay (ELISA) and immunoblot assay. The influence of Hed on cell motility angiogenesis was evaluated by Transwell and tube formation assays, while immunoblot analysis was used to determine the mechanism of action of Hed.Results: Hed inhibited the viability of RA-FLS cells and suppressed the inflammation of RA-FLS cells (p < 0.05). Furthermore, Hed suppressed the migration and angiogenesis of RA-FLSl cells, as well as regulated MAPK pathway (p < 0.05).Conclusion: Hed inhibits the proliferation, angiogenesis and inflammation of fibroblast-like synovial cells in RA by regulating MAPK pathway. Therefore, Hed is a drug for the treatment of RA, However, in vivo studies to validate these findings are recommended.

引用

页码：2047 / 2051

页数：5