miR-29a-3p Regulates Autophagy by Targeting Akt3-Mediated mTOR in SiO2-Induced Lung Fibrosis

被引:15
|
作者
Li, Peiyuan [1 ]
Hao, Xiaohui [1 ,2 ]
Liu, Jiaxin [1 ]
Zhang, Qinxin [1 ]
Liang, Zixuan [1 ]
Li, Xinran [1 ]
Liu, Heliang [1 ,2 ]
机构
[1] North China Univ Sci & Technol, Sch Publ Hlth, Tangshan 063210, Peoples R China
[2] North China Univ Sci & Technol, Hebei Key Lab Organ Fibrosis, Tangshan 063210, Peoples R China
关键词
silicosis; lung epithelial cell; autophagy; miRNA microarray analysis; SILICOSIS; INFLAMMATION; MACROPHAGES; REVEALS; AKT1;
D O I
10.3390/ijms241411440
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Silicosis is a refractory pneumoconiosis of unknown etiology that is characterized by diffuse lung fibrosis, and microRNA (miRNA) dysregulation is connected to silicosis. Emerging evidence suggests that miRNAs modulate pulmonary fibrosis through autophagy; however, its underlying molecular mechanism remains unclear. In agreement with miRNA microarray analysis, the qRT-PCR results showed that miR-29a-3p was significantly decreased in the pulmonary fibrosis model both in vitro and in vivo. Increased autophagosome was observed via transmission electron microscopy in lung epithelial cell models and lung tissue of silicosis mice. The expression of autophagy-related proteins LC3 & alpha;/& beta; and Beclin1 were upregulated. The results from using 3-methyladenine, an autophagy inhibitor, or rapamycin, an autophagy inducer, together with TGF-& beta;1, indicated that autophagy attenuates fibrosis by protecting lung epithelial cells. In TGF-& beta;1-treated TC-1 cells, transfection with miR-29a-3p mimics activated protective autophagy and reduced alpha-smooth muscle actin and collagen I expression. miRNA TargetScan predicted, and dual-luciferase reporter experiments identified Akt3 as a direct target of miR-29a-3p. Furthermore, Akt3 expression was significantly elevated in the silicosis mouse model and TGF-& beta;1-treated TC-1 cells. The mammalian target of rapamycin (mTOR) is a central regulator of the autophagy process. Silencing Akt3 inhibited the transduction of the mTOR signaling pathway and activated autophagy in TGF-& beta;1-treated TC-1 cells. These results show that miR-29a-3p overexpression can partially reverse the fibrotic effects by activating autophagy of the pulmonary epithelial cells regulated by the Akt3/mTOR pathway. Therefore, targeting miR-29a-3p may provide a new therapeutic strategy for silica-induced pulmonary fibrosis.
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页数:16
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