Surveillance for SARS-CoV-2 and its variants in wastewater of tertiary care hospitals correlates with increasing case burden and outbreaks

被引：16

作者：

Acosta, Nicole ^{[1
]}

Bautista, Maria A. ^{[2
]}

Waddell, Barbara J. ^{[1
]}

Du, Kristine ^{[1
]}

McCalder, Janine ^{[1
,2
]}

Pradhan, Puja ^{[1
,2
]}

Sedaghat, Navid ^{[1
,2
]}

Papparis, Chloe ^{[1
,2
]}

Beaudet, Alexander Buchner ^{[1
]}

Chen, Jianwei ^{[2
]}

Van Doorn, Jennifer ^{[2
]}

Xiang, Kevin ^{[2
]}

Chan, Leslie ^{[2
]}

Vivas, Laura ^{[2
]}

Low, Kashtin ^{[1
]}

Lu, Xuewen ^{[3
]}

Lee, Jangwoo ^{[1
]}

Westlund, Paul ^{[4
]}

Chekouo, Thierry ^{[3
,5
]}

Dai, Xiaotian ^{[3
]}

Cabaj, Jason ^{[6
,7
,8
,9
,10
]}

Bhatnagar, Srijak ^{[11
]}

Ruecker, Norma ^{[12
]}

Achari, Gopal ^{[13
]}

Clark, Rhonda G. ^{[2
]}

Pearce, Craig ^{[14
]}

Harrison, Joe J. ^{[2
,8
,15
]}

Meddings, Jon ^{[7
,8
]}

Leal, Jenine ^{[1
,6
,10
,14
]}

Ellison, Jennifer ^{[14
]}

Missaghi, Bayan ^{[7
,8
,14
]}

Kanji, Jamil N. ^{[7
,8
,16
,17
,18
]}

Larios, Oscar ^{[7
,8
,14
,17
]}

Rennert-May, Elissa ^{[1
,6
,7
,8
,10
,15
]}

Kim, Joseph ^{[7
,8
,14
]}

Hrudey, Steve E. ^{[16
,19
]}

Lee, Bonita E. ^{[20
,21
,22
]}

Pang, Xiaoli ^{[16
,17
,22
]}

Frankowski, Kevin ^{[23
]}

Conly, John ^{[1
,7
,8
,10
,14
,15
,18
]}

Hubert, Casey R. J. ^{[2
]}

Parkins, Michael D. ^{[1
,7
,8
,15
]}

机构：

[1] Univ Calgary, Dept Microbiol Immunol & Infect Dis, Calgary, AB, Canada

[2] Univ Calgary, Dept Biol Sci, Calgary, AB, Canada

[3] Univ Calgary, Dept Math & Stat, Calgary, AB, Canada

[4] CEC Analyt Ltd, Calgary, AB, Canada

[5] Univ Minnesota, Sch Publ Hlth, Div Biostat, Minneapolis, MN USA

[6] Univ Calgary, Dept Community Hlth Sci, Calgary, AB, Canada

[7] Univ Calgary, Dept Med, Calgary, AB, Canada

[8] Alberta Hlth Serv, Calgary, AB, Canada

[9] Alberta Hlth Serv, Prov Populat & Publ Hlth, Calgary, AB, Canada

[10] Univ Calgary, OBrien Inst Publ Hlth, Calgary, AB, Canada

[11] Athabasca Univ, Fac Sci & Technol, Athabasca, AB, Canada

[12] Water Serv, Calgary, AB, Canada

[13] Univ Calgary, Dept Civil Engn, Calgary, AB, Canada

[14] Alberta Hlth Serv, Infect Prevent & Control, Calgary, AB, Canada

[15] Univ Calgary, Snyder Inst Chron Dis, Calgary, AB, Canada

[16] Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB, Canada

[17] Alberta Hlth Serv, Alberta Precis Labs, Publ Hlth Lab, Edmonton, AB, Canada

[18] Univ Calgary, Dept Pathol & Lab Med, Calgary, AB, Canada

[19] Univ Alberta, Dept Analyt & Environm Toxicol, Edmonton, AB, Canada

[20] Univ Alberta, Dept Pediat, Edmonton, AB, Canada

[21] Women & Childrens Hlth Res Inst, Edmonton, AB, Canada

[22] Univ Alberta, Li Ka Shing Inst Virol, Edmonton, AB, Canada

[23] Univ Calgary, Adv Canadian Water Assets, Calgary, AB, Canada

来源：

JOURNAL OF MEDICAL VIROLOGY | 2023年 / 95卷 / 02期

关键词：

COVID-19; hospital-acquired infection; prevalent; RT-qPCR; variant of concern; wastewater-based surveillance;

D O I：

10.1002/jmv.28442

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Wastewater-based SARS-CoV-2 surveillance enables unbiased and comprehensive monitoring of defined sewersheds. We performed real-time monitoring of hospital wastewater that differentiated Delta and Omicron variants within total SARS-CoV-2-RNA, enabling correlation to COVID-19 cases from three tertiary-care facilities with >2100 inpatient beds in Calgary, Canada. RNA was extracted from hospital wastewater between August/2021 and January/2022, and SARS-CoV-2 quantified using RT-qPCR. Assays targeting R203M and R203K/G204R established the proportional abundance of Delta and Omicron, respectively. Total and variant-specific SARS-CoV-2 in wastewater was compared to data for variant specific COVID-19 hospitalizations, hospital-acquired infections, and outbreaks. Ninety-six percent (188/196) of wastewater samples were SARS-CoV-2 positive. Total SARS-CoV-2 RNA levels in wastewater increased in tandem with total prevalent cases (Delta plus Omicron). Variant-specific assessments showed this increase to be mainly driven by Omicron. Hospital-acquired cases of COVID-19 were associated with large spikes in wastewater SARS-CoV-2 and levels were significantly increased during outbreaks relative to nonoutbreak periods for total SARS-CoV2, Delta and Omicron. SARS-CoV-2 in hospital wastewater was significantly higher during the Omicron-wave irrespective of outbreaks. Wastewater-based monitoring of SARS-CoV-2 and its variants represents a novel tool for passive COVID-19 infection surveillance, case identification, containment, and potentially to mitigate viral spread in hospitals.

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