The COMPASS Complex Regulates Fungal Development and Virulence through Histone Crosstalk in the Fungal Pathogen Cryptococcus neoformans

被引：5
作者：
Liu, Ruoyan [1 ]
Chen, Xiaoyu [1 ]
Zhao, Fujie [1 ]
Jiang, Yixuan [1 ]
Lu, Zhenguo [1 ]
Ji, Huining [2 ]
Feng, Yuanyuan [2 ]
Li, Junqiang [1 ]
Zhang, Heng [1 ]
Zheng, Jianting [2 ,3 ]
Zhang, Jing [1 ]
Zhao, Youbao [1 ]
机构：
[1] Henan Agr Univ, Coll Vet Med, Zhengzhou 450046, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
[3] Shanghai Jiao Tong Univ, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China
来源：
JOURNAL OF FUNGI | 2023年 / 9卷 / 06期
基金：
中国国家自然科学基金;
关键词：
COMPASS; H2B ubiquitination; Cryptococcus neoformans; yeast-to-hypha transition; virulence; H3K4; METHYLATION; HYPHAL GROWTH; C; NEOFORMANS; TITAN CELL; METHYLTRANSFERASE; TERMINATION; HOMOLOG; FAMILY; DOMAIN; LEVEL;
D O I：
10.3390/jof9060672
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The Complex of Proteins Associated with Set1 (COMPASS) methylates lysine K4 on histone H3 (H3K4) and is conserved from yeast to humans. Its subunits and regulatory roles in the meningitis-causing fungal pathogen Cryptococcus neoformans remain unknown. Here we identified the core subunits of the COMPASS complex in C. neoformans and C. deneoformans and confirmed their conserved roles in H3K4 methylation. Through AlphaFold modeling, we found that Set1, Bre2, Swd1, and Swd3 form the catalytic core of the COMPASS complex and regulate the cryptococcal yeast-to-hypha transition, thermal tolerance, and virulence. The COMPASS complex-mediated histone H3K4 methylation requires H2B mono-ubiquitination by Rad6/Bre1 and the Paf1 complex in order to activate the expression of genes specific for the yeast-to-hypha transition in C. deneoformans. Taken together, our findings demonstrate that putative COMPASS subunits function as a unified complex, contributing to cryptococcal development and virulence.
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页数：21
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