Bovine blastocyst-like structures derived from stem cell cultures

被引：38

作者：

Pinzon-Arteaga, Carlos A. ^{[1
]}

Wang, Yinjuan ^{[2
]}

Wei, Yulei ^{[1
,3
]}

Orsi, Ana E. Ribeiro ^{[1
,5
]}

Li, Leijie ^{[4
]}

Scatolin, Giovanna ^{[2
,6
]}

Liu, Lizhong ^{[1
]}

Sakurai, Masahiro ^{[1
]}

Ye, Jianfeng ^{[7
]}

Ming, Hao ^{[2
]}

Yu, Leqian ^{[1
,8
,9
]}

Li, Bo ^{[7
]}

Jiang, Zongliang ^{[2
,6
,10
]}

Wu, Jun ^{[1
,11
,12
]}

机构：

[1] Univ Texas Southwestern Med Ctr, Dept Mol Biol, Dallas, TX 75390 USA

[2] Louisiana State Univ, Sch Anim Sci, AgCenter, Baton Rouge, LA 70810 USA

[3] China Agr Univ, Coll Biol Sci, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, SJTU Yale Joint Ctr Biostat & Data Sci, Shanghai, Peoples R China

[5] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Sao Paulo, Brazil

[6] Univ Florida, Genet Inst, Dept Anim Sci, Gainesville, FL 32610 USA

[7] Univ Texas Southwestern Med Ctr, Lyda Hill Dept Bioinformat, Dallas, TX USA

[8] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China

[9] Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing 100101, Peoples R China

[10] Univ Florida, Genet Inst, Gainesville, FL 32610 USA

[11] Univ Texas Southwestern Med Ctr, Hamon Ctr Regenerat Sci & Med, Dallas, TX 75390 USA

[12] Univ Texas Southwestern Med Ctr, Cecil H & Ida Green Ctr Reprod Biol Sci, Dallas, TX 75390 USA

来源：

CELL STEM CELL | 2023年 / 30卷 / 05期

基金：

瑞典研究理事会; 美国农业部; 美国国家卫生研究院; 巴西圣保罗研究基金会;

关键词：

GENE-EXPRESSION; DERIVATION;

D O I：

10.1016/j.stem.2023.04.003

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogen-esis and improving reproductive efficiency in livestock species.

引用

页码：611 / +

页数：14

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