Enterobacteria in anaerobic digestion of dairy cattle wastewater: Assessing virulence and resistance for one health security

被引：2

作者：

Menezes, kassia Vidal ^{[1
]}

Duarte, Carlos Eduardo de Souza ^{[2
]}

Moreira, Myleny Goularte ^{[2
]}

Moreno, Talita de Jesus Cattem ^{[2
]}

Pereira, Vitor Jose da Silva ^{[2
]}

Ucella-Filho, Joao Gilberto Meza ^{[3
]}

Otenio, Marcelo Henrique ^{[4
]}

Ignacchiti, Mariana Drummond Costa ^{[2
]}

Resende, Juliana Alves ^{[1
,2
]}

机构：

[1] Fed Univ Espirito Santo UFES, Ctr Agr Sci & Engn CCAE, Grad Program Vet Sci, Alto Univ S-N, BR-29500000 Alegre, ES, Brazil

[2] Fed Univ Espirito Santo UFES, Dept Pharm & Nutr, Alto Univ S-N, BR-29500000 Alegre, ES, Brazil

[3] Fed Univ Espirito Santo UFES, Dept Forestry & Wood Sci, BR-29550000 Jeronimo Monteiro, ES, Brazil

[4] Brazilian Agr Res Corp, Embrapa Dairy Cattle, BR-36038330 Juiz De Fora, Brazil

来源：

WATER RESEARCH | 2024年 / 252卷

关键词：

Biocides; Biofilm; E; coli; Multidrug-resistant phenotypes; Biodigestion; ANTIBIOTIC-RESISTANCE; COW MANURE; E; COLI; PATHOGENS; GENES; INACTIVATION; RELEVANCE;

D O I：

10.1016/j.watres.2024.121192

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Samples from a dairy cattle waste -fed anaerobic digester were collected across seasons to assess sanitary safety for biofertilizer use. Isolated enterobacteria (suggestive of Escherichia coli) were tested for susceptibility to biocides, antimicrobials, and biofilm-forming capability. Results revealed a decrease in total bacteria, coliforms, and enterobacteria in biofertilizer compared to the effluent. Among 488 isolates, 98.12 % exhibited high biofilm formation. Biofertilizer isolates exhibited a similar biofilm formation capability as effluent isolates in summer, but greater propensity in winter. Resistance to biocides and antimicrobials varied, with tetracycline resistance reaching 19 %. Of the isolates, 25 were multidrug-resistant (MDR), with 64 % resistant to three drugs. Positive correlations were observed between MDR and increased biofilm formation capacity in both samples, while there was negative correlation between MDR and increased biocide resistance. A higher number of MDR bacteria were found in biofertilizer compared to the effluent, revealing the persistence of E. coli resistance, posing challenges to food safety and public health.

引用

页数：11

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