SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

被引：1
作者：
Li, Qianqian [1 ,2 ]
Tang, Maolin [1 ,2 ]
Zhao, Shisheng [1 ,2 ]
Yang, Junjie [1 ]
Meng, Yuanlin [1 ]
Meng, Chunmei [2 ]
Ren, Ling [2 ]
Hu, Weimin [1 ,2 ]
机构：
[1] North Sichuan Med Coll, Inst Basic Med & Forens Med, Nanchong 637100, Peoples R China
[2] North Sichuan Med Coll, Dept Immunol, Nanchong 637100, Peoples R China
来源：
DISCOVER ONCOLOGY | 2024年 / 15卷 / 01期
关键词：
Serum amyloid A1 (SAA1); Esophageal squamous cell carcinoma (ESCC); pSer675-beta-catenin; S1P/S1PR1; SQUAMOUS-CELL CARCINOMA; SPHINGOSINE; 1-PHOSPHATE; CANCER CELLS; EXPRESSION; APOPTOSIS;
D O I：
10.1007/s12672-024-00923-3
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Serum amyloid A1 (SAA1), an inflammation-related molecule, is associated with the malignant progression of many tumors. This study aimed to investigate the role of SAA1 in the progression of esophageal squamous cell carcinoma (ESCC) and its molecular mechanisms. The expression of SAA1 in ESCC tissues and cell lines was analyzed using bioinformatics analysis, western blotting, and reverse transcription-quantitative PCR (RT-qPCR). SAA1-overexpressing or SAA1-knockdown ESCC cells were used to assess the effects of SAA1 on the proliferation, migration, apoptosis of cancer cells and the growth of xenograft tumors in nude mice. Western blotting, immunofluorescence and RT-qPCR were used to investigate the relationship between SAA1 and beta-catenin and SAA1 and sphingosine 1-phosphate (S1P)/sphingosine 1-phosphate receptor 1 (S1PR1). SAA1 was highly expressed in ESCC tissues and cell lines. Overexpression of SAA1 significantly promoted the proliferation, migration and the growth of tumors in nude mice. Knockdown of SAA1 had the opposite effects and promoted the apoptosis of ESCC cells. Moreover, SAA1 overexpression promoted the phosphorylation of beta-catenin at Ser675 and increased the expression levels of the beta-catenin target genes MYC and MMP9. Knockdown of SAA1 had the opposite effects. S1P/S1PR1 upregulated SAA1 expression and beta-catenin phosphorylation at Ser675 in ESCC cells. In conclusion, SAA1 promotes the progression of ESCC by increasing beta-catenin phosphorylation at Ser675, and the S1P/S1PR1 pathway plays an important role in its upstream regulation.
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页数：14
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