UHPLC-Q-TOF analysis of pyrrolizidine alkaloids in North-Macedonian honey

被引：2

作者：

Cvetanoska, Marinela ^{[1
]}

Pocrnic, Marijana ^{[2
]}

Stefova, Marina ^{[1
]}

Galic, Nives ^{[2
]}

Stanoeva, Jasmina Petreska ^{[1
]}

机构：

[1] Ss Cyril & Methodius Univ, Inst Chem, Fac Nat Sci & Math, R N Macedonia, Skopje, North Macedonia

[2] Univ Zagreb, Fac Sci, Dept Chem, Zagreb, Croatia

来源：

FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE | 2024年 / 17卷 / 01期

关键词：

Pyrrolizidine alkaloids (PAs); UHPLC-Q-TOF; honey; food safety; extraction efficiency; clean-up; SOLID-PHASE EXTRACTION; LC-MS ANALYSIS; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; ECHIUM-VULGARE; VALIDATION; HEALTH; RISK;

D O I：

10.1080/19393210.2023.2266701

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Honey contaminated with pyrrolizidine alkaloids (PAs) could pose a risk for human consumption, being a widely consumed food product. A fast and simple LC/MS method for the analysis of pyrrolizidine alkaloids in honey was optimised to collect occurrence data. The extraction efficiency was evaluated by a systematic study of multiple solvent mixtures and clean-up procedures. The best results for PA extraction were obtained using a formic acid/methanol mixture with subsequent clean-up by the QuEChERS method, resulting in a mean recovery range of 91.8-102%. The method validation showed satisfactory intra-day (RSD < 5.1%) and inter-day precision (RSD < 9.1%). The proposed method was applied to 14 samples. A total of six PAs and two N-oxides were detected, with levels between 89 and 8188 mu g/kg. This assessment highlights the potential risk of intoxication and the need for further investigations regarding an effective quality system for manufacturers to control PAs in honey.

引用

页码：5 / 15

页数：11

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