Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction

被引：4

作者：

Thaler, Catherine D. ^{[1
]}

Carstens, Kaira ^{[2
]}

Martinez, Gabrielle ^{[3
]}

Stephens, Kimberly ^{[3
]}

Cardullo, Richard A. ^{[1
,2
,3
]}

机构：

[1] Univ Calif Riverside, Dept Evolut Ecol & Organismal Biol, Riverside, CA USA

[2] Univ Calif Riverside, Dept Biochem, Riverside, CA USA

[3] Univ Calif Riverside, Dept Entomol, Riverside, CA USA

来源：

PLOS ONE | 2023年 / 18卷 / 02期

关键词：

SECRETORY PATHWAY CA2+-ATPASE; IPG STRIP PREFRACTIONATION; IMPORTIN-ALPHA; NUCLEOCYTOPLASMIC TRANSPORT; NUCLEAR TRANSPORT; AXONEMAL DYNEIN; FLAGELLAR BEAT; SEMINAL FLUID; MOUSE SPERM; CALCIUM;

D O I：

10.1371/journal.pone.0280013

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mature sperm from Culex pipiens were isolated and analyzed by mass spectrometry to generate a mature sperm proteome dataset. In this study, we highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm. The proteome includes 1700 unique protein IDs, including a number of uncharacterized proteins. Here we discuss those proteins that may contribute to the unusual structure of the Culex sperm flagellum, as well as potential regulators of calcium mobilization and phosphorylation pathways that regulate motility. This database will prove useful for understanding the mechanisms that activate and maintain sperm motility as well as identify potential molecular targets for mosquito population control.

引用

页数：24