3D-Printed Hydrodynamic Focusing Lab-on-a-Chip Device for Impedance Flow Particle Analysis

被引：5

作者：

Desagani, Dayananda ^{[1
]}

Kleiman, Shani ^{[1
]}

Zagardan, Teddy ^{[1
]}

Ben-Yoav, Hadar ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Ilse Katz Inst Nanoscale Sci & Technol, Dept Biomed Engn, Nanobioelectron Lab, IL-8410501 Beer Sheva, Israel

来源：

CHEMOSENSORS | 2023年 / 11卷 / 05期

关键词：

lab-on-a-chip; 3D printing; point-of-care detection; electrochemical impedance spectroscopy; microfluidics; rapid prototyping; fused filament fabrication; flow cytometry; flow focusing; finite element method modeling; SPECTROSCOPY; ELECTRODES; CYTOMETRY;

D O I：

10.3390/chemosensors11050283

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Particles analysis, such as cell counting and differentiation, are widely used for the diagnosis and monitoring of several medical conditions, such as during inflammation. Three-dimensional-printed lab-on-a-chip (LOC) devices, which can utilize one of the cell counting methods, can bring this technology to remote locations through its cost-efficient advantages and easy handling. We present a three-dimensional-printed LOC device with integrated electrodes. To overcome the limited resolution of a 3D printer, we utilized a flow-focusing design. We modeled and simulated the mass transfer and flow dynamics in the LOC by incorporating a flow-focusing design and reached an optimal channel diameter of 0.5 mm, resulting in a flow-focusing distance of <60 mu m. We also used electrochemical impedance spectroscopy to enable the dependence of the electrode-solution interface on the flow-focusing properties. Finally, we highlighted the proof-of-concept detection of microspheres (6 mu m diameter), which model biological cells that flow in the channel, by recording the electrochemical impedance at 10 kHz, thus showing the potential of a future point-of-care (POC) device.

引用

页数：12