Comprehensive in silico analysis of the probiotics, and preparation of compound probiotics-Polygonatum sibiricum saponin with hypoglycemic properties

被引：12

作者：

Luo, Jiayuan ^{[1
]}

Jiang, Lu ^{[1
]}

Gao, Bo ^{[1
]}

Chai, Yangyang ^{[1
,2
]}

Bao, Yihong ^{[1
,2
]}

机构：

[1] Northeast Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China

[2] Key Lab Forest Food Resources Utilizat Heilongjian, Harbin 150040, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 404卷

基金：

黑龙江省自然科学基金; 中国博士后科学基金; 中国国家自然科学基金;

关键词：

Probiotics; Screening; Polygonatum sibiricum; Saponin; Hypoglycemic; Fecal fermentation; LACTOBACILLUS; FERMENTATION; ADHESION; FOOD;

D O I：

10.1016/j.foodchem.2022.134569

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, a combined application of Polygonatum sibiricum saponin (PSS) with probiotics was developed as a new formulation that could be a candidate for a dietary supplement. The properties of nine probiotics were evaluated by principal component and heatmap analysis. And the hypoglycemic properties of compound pro-biotics were compared with single strains. The results showed the inhibition (%) of alpha-amylase was higher when L. casei ATCC393 was used synergistically with L. Bulgaricus 1.1480 compared with single strains as well as other strain combinations. And it was also found that the inhibition (%) of alpha-amylase was higher as 70.35 % after PSS and the compound probiotic were compounded as a ratio of 2:1. Furturemore, PSS-compound probiotics could regulate the composition of the gut microbiome of T2DM mice and enhance the metabolic capacity. In conclusion, the combination of PSS and compound probiotics has shown massive potential as management nutraceuticals of T2DM.

引用

页数：11