Polydatin nanoparticles attenuate oxidative stress and histopathological changes in streptozotocin model of diabetic nephropathy: targeting Nrf2/HO-1/NF-κβ signaling pathways

被引：4

作者：

Abdul-Hamid, Manal ^{[1
]}

Galaly, Sanaa R. ^{[1
]}

Mohamed, Hanaa M. ^{[2
]}

Mostafa, Fatma ^{[1
]}

Abdel-Moneim, Adel ^{[3
]}

机构：

[1] Beni Suef Univ, Fac Sci, Zool Dept, Histol & Cytol Div, Salah Salem St,POB 62521, Bani Suwayf 62521, Egypt

[2] Beni Suef Univ, Fac Sci, Zool Dept, Genet & Mol Genet Div, Bani Suwayf, Egypt

[3] Beni Suef Univ, Fac Sci, Zool Dept, Mol Physiol Div, Bani Suwayf, Egypt

来源：

BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES | 2023年 / 12卷 / 01期

关键词：

Diabetic nephropathy; Metformin; Polydatin nanoparticles; Oxidative stress biomarkers; Histopathology; Ultrastructure; NF-KAPPA-B; RECEPTOR; PATHOGENESIS; ANTIOXIDANT; PEROXIDASE; ACTIVATION; INDUCTION; CATALASE; KIDNEY; INJURY;

D O I：

10.1186/s43088-023-00441-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

BackgroundOne of the most prevalent and serious side effects of diabetes mellitus is diabetic nephropathy, which is characterized by abnormalities in kidney structure that can occur before kidney function declines. Up to 90% of persons with diabetic nephropathy and 40% of adults with severe diabetic nephropathy are unaware that they have kidney disease. Through Nrf2/HO-1/NF kappa beta/TNF alpha signaling pathways, biochemical, oxidative stress, and antioxidant biomarkers, ultrastructural, immunohistochemical, and histopathological studies, so we attempt to evaluate the potential corrective mechanisms of polydatin nanoparticles against diabetic nephropathy in comparison with polydatin and metformin.ResultsPOLY-CSNPs, POLY, and METF treatment lowered fasting superoxide dismutase, catalase activities, and glutathione content in treating the diabetic blood sugar level, glycosylated hemoglobin percentage, and oxidative stress such as lipid peroxidation level, and also enhanced antioxidant biomarkers like superoxide peroxidase, superoxide dismutase, catalase activities, and glutathione content in treating the diabetic nephropathy. POLY-CSNPs, POLY, and METF also significantly reduced the area % of immunohistochemical reaction of TNF alpha (tumor necrosis factor alpha) and NF kappa beta (nuclear factor-kappa-beta), while significantly increasing the mRNA expression's levels for nuclear factor erythroid 2-related factor 2 and heme oxygenase-1. The results observed that POLY-CSNPs showed extremely significant efficacy in treated diabetic rats as contrasted with POLY. Histological and ultrastructural studies showed marked improvement in glomeruli, basal laminae, and proximal tubules appearing nearly identical to the normal.ConclusionsPOLY-CSNPs revealed a marked ameliorative effect on diabetic nephropathy via its anti-inflammatory, antioxidant, and prolonged-release properties.

引用

页数：20

共 77 条

[71] Potential therapeutic effects of the simultaneous targeting of the Nrf2 and NF-κB pathways in diabetic neuropathy [J].