Life-cycle selenium accumulation and its correlations with the rhizobacteria and endophytes in the hyperaccumulating plant Cardamine hupingshanensis

被引：5

作者：

Zang, Huawei ^{[1
,2
,3
]}

Tong, Xinzhao ^{[4
]}

Yuan, Linxi ^{[3
]}

Zhang, Ying ^{[5
]}

Zhang, Ru ^{[6
]}

Li, Miao ^{[7
]}

Zhu, Renbin ^{[1
,2
]}

机构：

[1] Univ Sci & Technol China, Inst Polar Environm, Hefei 230026, Peoples R China

[2] Univ Sci & Technol China, Sch Earth & Space Sci, Anhui Prov Key Lab Polar Environm & Global Change, Hefei 230026, Peoples R China

[3] Xian Jiaotong Liverpool Univ, Dept Hlth & Environm Sci, Suzhou 215123, Jiangsu, Peoples R China

[4] Xian Jiaotong Liverpool Univ, Dept Biol Sci, Suzhou 215123, Jiangsu, Peoples R China

[5] Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou 215123, Peoples R China

[6] Anhui Agr Univ, Sch Resources & Environm, Hefei 230036, Peoples R China

[7] Anhui Agr Univ, Sch Plant Protect, Anhui Prov Key Lab Crop Integrated Pest Management, Anhui Prov Engn Lab Green Pesticide Dev & Applicat, Hefei 230036, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2023年 / 264卷

基金：

中国国家自然科学基金;

关键词：

Cardamine hupingshanensis; Se hyperaccumulator; Rhizosphere soil; Rhizobacteria; Endophyte; PHOSPHATE TRANSPORTER; GAMMA-IRRADIATION; ARABIDOPSIS; PHYTOREMEDIATION; BIOFORTIFICATION; SULFATE; GROWTH; OVEREXPRESSION; MICROBIOME; MECHANISMS;

D O I：

10.1016/j.ecoenv.2023.115450

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Cardamine hupingshanensis (C. hupingshanensis) is known for its ability to hyperaccumulate selenium (Se). However, the roles of the rhizobacteria or endophytes in Se hyperaccumulation have not been explored in C. hupingshanensis. Here, in-situ-like pot experiments were conducted to investigate the characteristics of Se accumulation throughout C. hupingshanensis growth stages and its correlations with rhizobacteria and endophytes under varying soil Se levels. Results showed that Se levels in roots, stems and leaves increased from the seedling to bolting stage, but remained relatively stable during the flowering and maturity. Leaves exhibited the highest Se levels (736.48 +/- 6.51 mg/kg DW), followed by stems (575.39 +/- 27.05 mg/kg DW), and lowest in roots (306.62 +/- 65.45 mg/kg DW) under high-Se stress. The Se translocation factors from soils to C. hupingshanensis roots was significantly higher (p < 0.05) in low-Se soils compared to medium- and high-Se soils. Rhizobacterial diversity showed significant positive correlations (p < 0.05) with both total and bioavailable soil Se contents. The levels of soil Se and growth stages of C. hupingshanensis were found to have significant effects (p < 0.03) on the compositions of rhizosphere bacteria and C. hupingshanensis endophytes. Low-abundance bacteria (< 5%), including Gemmatimonadetes, Latescibacteria and Nitrospirae, were identified to potentially increase the bioavailable Se levels in the rhizosphere. The Se accumulation significantly decreased (p < 0.05) in C. hupingshanensis grown in sterilized low- (32.4%), medium- (17%) and high-Se (42%) soils. Endophytes in C. hupingshanensis, such as Firmicutes and Proteobacteria, were likely recruited from the rhizobacteria, as evidenced by the isolated bacterial strains, and played an important role in Se hyperaccumulation, particularly during the flowering stage. This study provides new insights into potential mechanism underlying Se hyperaccumulation in C. hupingshanensis.

引用

页数：11