Functional and pharmacological analyses of visual habituation learning in larval zebrafish

被引:3
作者
Lamire, Laurie Anne [1 ]
Haesemeyer, Martin [2 ]
Engert, Florian [3 ,4 ]
Granato, Michael [5 ]
Randlett, Owen [1 ]
机构
[1] UCBL, Inst NeuroMyoGene, Fac Med & Pharm, Lab MeLiS,CNRS,UMR5284,Inserm U1314, Lyon, France
[2] Ohio State Univ, Dept Neurosci, Columbus, OH USA
[3] Harvard Univ, Dept Mol & Cellular Biol, Fac Arts & Sci, Cambridge, MA USA
[4] Harvard Univ, Fac Arts & Sci, Ctr Brain Sci, Cambridge, MA USA
[5] Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA USA
基金
美国国家科学基金会;
关键词
behaviour; plasticity; habituation; Ca2+ imaging; pharmacology; GABA; Zebrafish; LONG-TERM HABITUATION; NEURAL ACTIVITY; BRAIN ACTIVITY; MELATONIN; MEMORY; PLASTICITY; BEHAVIOR; SCALE; GABA;
D O I
10.7554/eLife.84926
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Habituation allows animals to learn to ignore persistent but inconsequential stimuli. Despite being the most basic form of learning, a consensus model on the underlying mechanisms has yet to emerge. To probe relevant mechanisms, we took advantage of a visual habituation paradigm in larval zebrafish, where larvae reduce their reactions to abrupt global dimming (a dark flash). We used Ca2+ imaging during repeated dark flashes and identified 12 functional classes of neurons that differ based on their rate of adaptation, stimulus response shape, and anatomical location. While most classes of neurons depressed their responses to repeated stimuli, we identified populations that did not adapt or that potentiated their response. These neurons were distributed across brain areas, consistent with a distributed learning process. Using a small-molecule screening approach, we confirmed that habituation manifests from multiple distinct molecular mechanisms, and we have implicated molecular pathways in habituation, including melatonin, oestrogen, and GABA signalling. However, by combining anatomical analyses and pharmacological manipulations with Ca2+ imaging, we failed to identify a simple relationship between pharmacology, altered activity patterns, and habituation behaviour. Collectively, our work indicates that habituation occurs via a complex and distributed plasticity processes that cannot be captured by a simple model. Therefore, untangling the mechanisms of habituation will likely require dedicated approaches aimed at sub-component mechanisms underlying this multidimensional learning process.
引用
收藏
页数:25
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