What do oysters smell? Electrophysiological evidence that the bivalve osphradium is a chemosensory organ in the oyster, Magallana gigas

被引:5
作者
Rato, Ana [1 ,2 ]
Joaquim, Sandra [2 ,3 ]
Matias, Domitilia [2 ,3 ]
Hubbard, Peter C. [1 ]
机构
[1] Univ Algarve, Ctr Marine Sci CCMAR, Campus Gambelas, P-8005139 Faro, Portugal
[2] Portuguese Inst Sea & Atmosphere IPMA IP, Dept Sea & Marine Resources, Ave 5 Outubro S-N, P-8700305 Olhao, Portugal
[3] Univ Porto, Interdisciplinary Ctr Marine Environm Res CIIMAR, Ave Gen Norton de Matos S-N, P-4450208 Matosinhos, Portugal
来源
JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY | 2023年 / 209卷 / 03期
关键词
EOsG; Electrophysiology; Chemoreception; Bivalve; Amino acids; OLFACTORY SENSITIVITY; AMINO-ACIDS; FINE MORPHOLOGY; SENSE-ORGANS; LIVE MICROALGAE; PACIFIC OYSTER; RESPONSES; MOLLUSCA; SETTLEMENT; GABA;
D O I
10.1007/s00359-022-01608-4
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
The sensing of chemical cues is essential for several aspects of bivalve biology, such as the detection of food and pheromones. However, little is known about chemical communication systems in bivalves or the possible role of the osphradium as a chemosensory organ. To address this, we adapted an electrophysiological technique extensively used in vertebrates-the electro-olfactogram-to record from the osphradium in the Pacific oyster, Magallana gigas. This technique was validated using amino acids as stimulants. The osphradium proved to be sensitive to most proteinogenic l-amino acids tested, evoking tonic, negative, concentration-dependent 'electro-osphradiogram' (EOsG) voltage responses, with thresholds of detection in the range of 10(-6) to 10(- 5) M. Conversely, it was insensitive to l-arginine and l-glutamic acid. The current study supports the hypothesis that the osphradium is, indeed, a chemosensory organ. The 'electro-osphradiogram' may prove to be a powerful tool in the isolation and characterization of pheromones and other important chemical cues in bivalve biology.
引用
收藏
页码:391 / 401
页数:11
相关论文
共 63 条
[1]   Olfaction: Diverse species, conserved principles [J].
Ache, BW ;
Young, JM .
NEURON, 2005, 48 (03) :417-430
[2]   NERVOUS CONTROL OF CILIARY ACTIVITY [J].
AIELLO, E ;
GUIDERI, G .
SCIENCE, 1964, 146 (365) :1692-&
[3]   A non-invasive anaesthetic method for accessing the brood chamber of the Chilean flat oyster (Ostrea chilensis) [J].
Alipia, T. T. ;
Mae, H. ;
Dunphy, B. J. .
NEW ZEALAND JOURNAL OF MARINE AND FRESHWATER RESEARCH, 2014, 48 (03) :350-355
[4]   Feasibility of dietary substitution of live microalgae with spray-dried Schizochytrium sp or Spirulina in the hatchery culture of juveniles of the Pacific geoduck clam (Panopea generosa) [J].
Arney, Bianca ;
Liu, Wenshan ;
Forster, Ian P. ;
McKinley, R. Scott ;
Pearce, Christopher M. .
AQUACULTURE, 2015, 444 :117-133
[5]   OLFACTORY RESPONSES OF AQUATIC AND TERRESTRIAL TIGER SALAMANDERS TO AIRBORNE AND WATERBORNE STIMULI [J].
ARZT, AH ;
SILVER, WL ;
MASON, JR ;
CLARK, L .
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 1986, 158 (04) :479-487
[6]  
Atema J, 2012, CHEMICAL ECOLOGY IN AQUATIC SYSTEMS, P1
[7]   Beyond relaxed: magnesium chloride anaesthesia alters the circulatory metabolome of a marine mollusc (Perna canaliculus) [J].
Azizan, Awanis ;
Alfaro, Andrea C. ;
Young, Tim ;
Venter, Leonie .
METABOLOMICS, 2021, 17 (08)
[8]  
BAILEY DF, 1966, J EXP BIOL, V44, P131
[9]   THE OSPHRADIUM IN PLACOPECTEN-MAGELLANICUS AND PECTEN-MAXIMUS (BIVALVIA, PECTINIDAE) - HISTOLOGY, ULTRASTRUCTURE, AND IMPLICATIONS FOR SPAWNING SYNCHRONIZATION [J].
BENINGER, PG ;
DONVAL, A ;
LEPENNEC, M .
MARINE BIOLOGY, 1995, 123 (01) :121-129
[10]   Distance chemoreception in the common cuttlefish, Sepia officinalis (Mollusca, Cephalopoda) [J].
Boal, JG ;
Golden, DK .
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 1999, 235 (02) :307-317