Delineation of DNA and mRNA COVID-19 vaccine-induced immune responses in preclinical animal models

被引：6

作者：

Andrade, Viviane M. ^{[1
]}

Maricic, Igor ^{[1
]}

Kalia, Richa ^{[1
]}

Jachimowicz, Lauren ^{[1
]}

Bedoya, Olivia ^{[1
]}

Kulp, Daniel W. ^{[2
]}

Humeau, Laurent ^{[1
]}

Smith, Trevor R. F. ^{[1
,3
]}

机构：

[1] Inovio Pharmaceut Inc, Preclin R&D, San Diego, CA USA

[2] Wistar Inst Anat & Biol, Vaccine & Immunotherapy Ctr, Philadelphia, PA USA

[3] Inovio Pharmaceut Inc, Preclin R&D, 10480 Wateridge Circle, San Diego, CA 92121 USA

来源：

HUMAN VACCINES & IMMUNOTHERAPEUTICS | 2023年 / 19卷 / 03期

关键词：

DNA vaccines; mRNA vaccines; COVID-19; SARS-CoV-2; immunogenicity; animal models; T-CELL RESPONSES; GENE-TRANSFER; TRANSFECTION; INFECTION; PROSPECTS; DELIVERY; MUSCLE;

D O I：

10.1080/21645515.2023.2281733

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nucleic acid vaccines are designed based on genetic sequences (DNA or mRNA) of a target antigen to be expressed in vivo to drive a host immune response. In response to the COVID-19 pandemic, mRNA and DNA vaccines based on the SARS-CoV-2 Spike antigen were developed. Surprisingly, head-to-head characterizations of the immune responses elicited by each vaccine type has not been performed to date. Here, we have employed a range of preclinical animal models including the hamster, guinea pig, rabbit, and mouse to compare and delineate the immune response raised by DNA, administered intradermally (ID) with electroporation (EP) and mRNA vaccines (BNT162b2 or mRNA-1273), administered intramuscularly (IM), expressing the SARS-CoV-2 WT spike antigen. The results revealed clear differences in the quality and magnitude of the immune response between the two vaccine platforms. The DNA vaccine immune response was characterized by strong T cell responses, while the mRNA vaccine elicited robust humoral responses. The results may assist in guiding the disease target each vaccine type may be best matched against and suggest mechanisms to further enhance the breadth of each platform's immune response.

引用

页数：12

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