Effect of hydroxylated and methylated flavonoids on cytochrome P450 activity in porcine intestinal epithelial cells

被引:1
作者
Karancsi, Zita [1 ]
Kovacs, Dora [1 ]
Csiko, Gyorgy [1 ]
Palocz, Orsolya [1 ]
Jerzsele, Akos [1 ]
Galfi, Peter [1 ]
Farkas, Orsolya [1 ]
机构
[1] Univ Vet Med, Budapest, Hungary
关键词
flavonoids; apigenin; quercetin; IPEC-J2; LIVER-MICROSOMES; DIETARY FLAVONOIDS; DRUG-METABOLISM; IN-VIVO; INHIBITION; EXPRESSION; ABSORPTION; BIOAVAILABILITY; PREVENTION; MIDAZOLAM;
D O I
10.1556/004.2023.00746
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Cytochrome P450 (CYP) oxidases are among the main metabolizing enzymes that are responsible for the transformation of xenobiotics, including clinically important drugs. Their activity can be influenced by several compounds leading to decreased efficacy or increased toxicity of co-administered medicines. Flavonoids exert various beneficial effects on human and animal health; therefore they are used as food and feed supplements. However, they are also well-known for their CYP modulating potential. Since the amount of CYP enzymes is highest in the liver, interaction studies are mainly conducted in hepatocytes, however, CYP activity in the gastrointestinal tract is also remarkable. In this study, effects of apigenin (API), quercetin (QUE) and their methylated derivatives trimethylapigenin (TM-API), 3-O-methylquercetin (3M-QUE) and 30,7-di-O-methylquercetin (307DMQUE) on the CYP enzyme activity was examined in IPEC-J2 porcine intestinal epithelial cells. Potential food-drug interactions were studied using flavonoid treatment in combination with inducer and inhibitor compounds. API, TM-API, QUE and 3M-QUE significantly inhibited the CYP3A29 enzyme, while 307DM-QUE did not alter its activity. Enzyme inhibition has also been observed in case of some food-drug combinations. Our results support previous findings about CYP modulating effects of flavonoids and highlights the possibility of interactions when flavonoid-containing supplements are consumed during drug treatments.
引用
收藏
页码:16 / 24
页数:9
相关论文
共 44 条
  • [1] Dietary flavonoids in cancer therapy and prevention: Substrates and inhibitors of cytochrome P450 CYP1 enzymes
    Androutsopoulos, Vasilis P.
    Papakyriakou, Athanasios
    Vourloumis, Dionisios
    Tsatsakis, Aristidis M.
    Spandidos, Demetrios A.
    [J]. PHARMACOLOGY & THERAPEUTICS, 2010, 126 (01) : 9 - 20
  • [2] A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells
    Ayuso, Miriam
    Van Cruchten, Steven
    Van Ginneken, Chris
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (19) : 1 - 18
  • [3] Grapefruit juice-drug interactions
    Bailey, DG
    Malcolm, J
    Arnold, O
    Spence, JD
    [J]. BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1998, 46 (02) : 101 - 110
  • [4] Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations
    Brosnahan, Amanda J.
    Brown, David R.
    [J]. VETERINARY MICROBIOLOGY, 2012, 156 (3-4) : 229 - 237
  • [5] Functional cell models of the gut and their applications in food microbiology A review
    Cencic, Avrelija
    Langerholc, Tomaz
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2010, 141 : S4 - S14
  • [6] Egert S, 2011, ADV NUTR, V2, P8, DOI [10.3945/an.110.000026, 10.3945/an.110.000026.]
  • [7] In Vitro Gender-Dependent Inhibition of Porcine Cytochrome P450 Activity by Selected Flavonoids and Phenolic Acids
    Ekstrand, Bo
    Rasmussen, Martin Kroyer
    Woll, Felicia
    Zlabek, Vladimir
    Zamaratskaia, Galia
    [J]. BIOMED RESEARCH INTERNATIONAL, 2015, 2015
  • [8] Polymethoxyflavone Apigenin-Trimethylether Suppresses LPS-Induced Inflammatory Response in Nontransformed Porcine Intestinal Cell Line IPEC-J2
    Farkas, Orsolya
    Palocz, Orsolya
    Paszti-Gere, Erzsebet
    Galfi, Peter
    [J]. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2015, 2015
  • [9] Gálvez J, 2001, STUD NAT PROD CHEM, V25, P607
  • [10] Flavonoid-gastrointestinal mucus interaction and its potential role in regulating flavonoid bioavailability and mucosal biophysical properties.
    Gonzales, Gerard Bryan
    Van Camp, John
    Smagghe, Guy
    Raes, Katleen
    Mackie, Alan
    [J]. FOOD RESEARCH INTERNATIONAL, 2016, 88 : 342 - 347