3D bio scaffold support osteogenic differentiation of mesenchymal stem cells

被引:1
作者
Ramzan, Faiza [1 ]
Khalid, Shumaila [1 ]
Ekram, Sobia [1 ]
Salim, Asmat [1 ]
Frazier, Trivia [2 ]
Begum, Sumreen [3 ]
Mohiuddin, Omair A. [1 ]
Khan, Irfan [1 ]
机构
[1] Univ Karachi, Dr Panjwani Ctr Mol Med & Drug Res, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan
[2] Obatala Sci Inc, New Orleans, LA USA
[3] Sindh Inst Urol & Transplantat SIUT, Stem Cell Res Lab SCRL, Karachi, Pakistan
关键词
biomaterial; bone regeneration; decellularization; human umbilical cord tissue; hydrogel; tissue engineering; EXTRACELLULAR-MATRIX SCAFFOLDS; IN-VITRO; TISSUE; BONE; CARTILAGE;
D O I
10.1002/cbin.12131
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual-lineage (osteogenesis and chondrogenesis). A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration. Mesenchymal stem cells (MSCs) isolated from the umbilical cord were seeded with hydrogel for 28 days, and cell-hydrogel composites were cultured in basal and osteogenic media. Alizarin red staining, quantitative polymerase chain reaction, and immunofluorescent staining were used to confirm that the hydrogel was biocompatible and capable of inducing osteogenic differentiation in umbilical cord-derived MSCs. The findings demonstrate that human MSCs differentiated into an osteogenic lineage following 28 days of cultivation in basal and osteoinductive media. The expression was higher in the cell-hydrogel composites cultured in osteoinductive media, as evidenced by increased levels of messenger RNA and protein expression of osteogenic markers as compared to basal media cultured cell-hydrogel composites. Additionally, calcium deposits were also observed, which provide additional evidence of osteogenic differentiation. The findings demonstrate that the hydrogel is biocompatible with MSCs and possesses osteoinductive capability in vitro. It may be potentially useful for osteochondral regeneration.
引用
收藏
页码:594 / 609
页数:16
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