The aqueous root extract of Withania somnifera ameliorates LPS-induced inflammatory changes in the in vitro cell-based and mice models of inflammation

被引:5
|
作者
Sharma, Phulwanti Kumari [1 ]
Kumar, Lokesh [1 ]
Goswami, Yamini [1 ]
Pujani, Mukta [2 ]
Dikshit, Madhu [1 ,3 ]
Tandon, Ruchi [1 ]
机构
[1] Translat Hlth Sci & Technol Inst, Faridabad, India
[2] ESIC Med Coll & Hosp, Faridabad, India
[3] Cent Drug Res Inst, Pharmacol Div, Lucknow, India
关键词
Withania somnifera; cytokines; lung inflammation; PBMCs; TLR-4; LPS; COVID-19; LIPOPOLYSACCHARIDE; WITHAFERIN; COVID-19; ASTHMA;
D O I
10.3389/fphar.2023.1139654
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Introduction: Most critically ill COVID-19 patients have bronchitis, pneumonia, and acute respiratory distress syndrome (ARDS) due to excessive inflammatory conditions. Corticosteroids have largely been prescribed for the management of inflammation in these patients. However, long-term use of corticosteroids in patients with comorbidities such as metabolic, cardiovascular, and other inflammatory disorders is ideally not recommended due to safety issues. A potential and safer anti-inflammatory therapy is therefore the need of the hour. Withania somnifera (WS), a well-known herbal medicine used during the pandemic in India to prevent SARS-CoV2 infection, also possesses anti-inflammatory properties.Methods: In the present study, we, therefore, evaluated the effect of the aqueous extract of the roots of W. somnifera in the cell-based assays and in the experimental animal models of LPS-induced inflammation.Results: In the NCI-H460, A549 cells and human peripheral blood mononuclear cells (PBMCs) pre-treatment with W. somnifera reduced the LPS-induced expression of the pro-inflammatory cytokines. In addition, W. somnifera extract also showed potent anti-inflammatory activity in the lung tissues of BALB/c mice challenged intranasally with LPS. We observed a marked reduction in the neutrophil counts in the broncho-alveolar lavage (BAL) fluid, inflammatory cytokines, and fibrosis in the mice lungs pre-treated with W. somnifera. Results obtained thus suggest the potential utility of W. somnifera extract in reducing airway inflammation and recommend the clinical evaluation of W. somnifera extract in COVID-19 patients with a high propensity for lung inflammation.
引用
收藏
页数:10
相关论文
共 11 条
  • [1] Withania somnifera root extract prevents DMBA-induced squamous cell carcinoma of skin in Swiss albino mice
    Prakash, J
    Gupta, SK
    Dinda, AK
    NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2002, 42 (01): : 91 - 97
  • [2] Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPS-induced models
    Hong, Joung-Woo
    Yang, Ga-Eun
    Kim, Yoon Bum
    Eom, Seok Hyun
    Lew, Jae-Hwan
    Kang, Hee
    BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2012, 12
  • [3] Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPS-induced models
    Joung-Woo Hong
    Ga-Eun Yang
    Yoon Bum Kim
    Seok Hyun Eom
    Jae-Hwan Lew
    Hee Kang
    BMC Complementary and Alternative Medicine, 12
  • [4] Agrimonia pilosa Ledeb Root Extract: Anti-Inflammatory Activities of the Medicinal Herb in LPS-Induced Inflammation
    Kim, Da-Sol
    Park, Kyoung-Eun
    Kwak, Yeon-Ju
    Bae, Moon-Kyoung
    Bae, Soo-Kyung
    Jang, Ik-Soon
    Jang, Hye-Ock
    AMERICAN JOURNAL OF CHINESE MEDICINE, 2020, 48 (08): : 1875 - 1893
  • [5] Syzygium aromaticum aqueous extract inhibits human neutrophils myeloperoxidase and protects mice from LPS-induced lung inflammation
    Chniguir, Amina
    Zioud, Fatma
    Marzaioli, Viviana
    El-Benna, Jamel
    Bachoual, Rafik
    PHARMACEUTICAL BIOLOGY, 2019, 57 (01) : 56 - 64
  • [6] Anti-inflammatory effect of alcoholic extract of Entada pursaetha DC in LPS-induced inflammation in mice and RAW264.7 cells
    Kumari, Rashmi Rekha
    Lingaraju, Madhu Cholenahalli
    Gupta, Gaurav
    More, Amar Sunil
    Balaganur, Venkanna
    Kumar, Dhirendra
    Kumar, Pankaj
    Kumar, Dinesh
    Sharma, Anil Kuma
    Mishra, Santosh Kumar
    Tandan, Surendra Kumar
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2017, 54 (3-4): : 140 - 149
  • [7] Anti-Inflammatory Effects of Cicadidae Periostracum Extract and Oleic Acid through Inhibiting Inflammatory Chemokines Using PCR Arrays in LPS-Induced Lung inflammation In Vitro
    Hong, Jung-Hee
    Lee, Young-Cheol
    LIFE-BASEL, 2022, 12 (06):
  • [8] Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo
    Lu Wang
    Dongli Song
    Chuanyuan Wei
    Cheng Chen
    Yanwen Yang
    Xinyi Deng
    Jianying Gu
    Journal of Translational Medicine, 18
  • [9] Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo
    Wang, Lu
    Song, Dongli
    Wei, Chuanyuan
    Chen, Cheng
    Yang, Yanwen
    Deng, Xinyi
    Gu, Jianying
    JOURNAL OF TRANSLATIONAL MEDICINE, 2020, 18 (01)
  • [10] In vitro and in vivo experimental investigation of anti-inflammatory effects of Peucedanum japonicum aqueous extract by suppressing the LPS-induced NF-κB/MAPK JNK pathways
    Gil, Tae-Yong
    Jin, Bo-Ram
    Lee, Jong-Hyun
    An, Hyo-Jin
    AMERICAN JOURNAL OF CHINESE MEDICINE, 2022, 50 (08): : 2153 - 2169