Curcumin-Loaded Macrophage-Derived Exosomes Effectively Improve Wound Healing

被引：22
作者：
Li, Daoyong [1 ]
Zhang, Chuanjie [1 ]
Gao, Zhanshan [2 ]
Xia, Nan [2 ]
Wu, Chao [2 ]
Liu, Chang [3 ]
Tian, He [4 ]
Mei, Xifan [1 ]
机构：
[1] Jinzhou Med Univ, Affiliated Hosp 3, Dept Orthoped, Jinzhou 121000, Liaoning, Peoples R China
[2] Jinzhou Med Univ, Pharm Sch, Jinzhou 121000, Liaoning, Peoples R China
[3] Jinzhou Med Univ, Affiliated Hosp 1, Dept Endocrinol, Jinzhou 121000, Liaoning, Peoples R China
[4] Jinzhou Med Univ, Dept Histol & Embryol, Jinzhou 121000, Liaoning, Peoples R China
来源：
MOLECULAR PHARMACEUTICS | 2023年 / 20卷 / 09期
基金：
中国国家自然科学基金;
关键词：
curcumin; exosomes; wound healing; antioxidants; anti-inflammatory;
D O I：
10.1021/acs.molpharmaceut.3c00062
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
This study aims to investigate the potential therapeuticeffectof exosomes derived from macrophages loaded with curcumin (Exos-cur)on the healing of diabetic wounds. As a new type of biomaterial, Exos-curhas better stability, anti-inflammation, and antioxidation biologicalactivity. In in vitro experiments, Exos-cur can promote the proliferation,migration, and angiogenesis of HUVECs (human umbilical vein endothelialcells) while reducing the ROS (reactive oxygen species) produced byHUVECs induced by high glucose, regulating the mitochondrial membranepotential, reducing cell oxidative damage, and inhibiting oxidativestress and inflammation. In the in vivo experiment, the Exos-cur treatmentgroup had an increased percentage of wound closure and contractioncompared with the diabetic wound control group. Hematoxylin-eosinstaining (HE) and Masson staining showed that the Exos-cur treatmentgroup had more advanced re-epithelialization, and the generated maturegranulation tissue was rich in a large number of capillaries and newlydeposited collagen fibers. Western blot and immunofluorescence analysesshowed that Exos-cur can inhibit inflammation by activating the Nrf2/AREpathway, upregulate the expression of wound healing-related molecules,promote angiogenesis, and accelerate wound healing in diabetic rats.These results show that Exos-cur has a good therapeutic effect ondiabetic skin defects and provide experimental evidence for the potentialclinical benefits of Exos-cur.
引用
收藏
页码：4453 / 4467
页数：15
相关论文
共 42 条
[1]   Lupeol, a Dietary Triterpene, Enhances Wound Healing in Streptozotocin-Induced Hyperglycemic Rats with Modulatory Effects on Inflammation, Oxidative Stress, and Angiogenesis [J].
Beserra, Fernando Pereira ;
Vieira, Ana Julia ;
Sergio Gushiken, Lucas Fernando ;
de Souza, Eduardo Oliveira ;
Hussni, Maria Fernanda ;
Hussni, Carlos Alberto ;
Nobrega, Rafael Henrique ;
Monteiro Martinez, Emanuel Ricardo ;
Jackson, Christopher John ;
de Azevedo Maia, Gabriela Lemos ;
Rozza, Ariane Leite ;
Pellizzon, Claudia Helena .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2019, 2019
[2]   Simvastatin enhances VEGF production and ameliorates impaired wound healing in experimental diabetes [J].
Bitto, Alessandra ;
Minutoli, Letteria ;
Altavilla, Domenica ;
Polito, Francesca ;
Fiurnara, Tiziana ;
Marini, Herbert ;
Galeano, Mariarosaria ;
Calo, Margherita ;
Lo Cascio, Patrizia ;
Bonaiuto, Michele ;
Migliorato, Alba ;
Caputi, Achille P. ;
Squadrito, Francesco .
PHARMACOLOGICAL RESEARCH, 2008, 57 (02) :159-169
[3]   CCL4 Deletion Accelerates Wound Healing by Improving Endothelial Cell Functions in Diabetes Mellitus [J].
Chang, Ting-Ting ;
Chen, Ching ;
Lin, Liang-Yu ;
Chen, Jaw-Wen .
BIOMEDICINES, 2022, 10 (08)
[4]   Encapsulation of green tea polyphenol nanospheres in PVA/alginate hydrogel for promoting wound healing of diabetic rats by regulating PI3K/AKT pathway [J].
Chen, Guanyu ;
He, Libang ;
Zhang, Peng ;
Zhang, Jie ;
Mei, Xifan ;
Wang, Dahao ;
Zhang, Yiyao ;
Ren, Xiuli ;
Chen, Zhenhua .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2020, 110
[5]   Evaluation of curcumin-loaded polymeric nanocapsules with different coatings in chick embryo model: influence on angiogenesis, teratogenesis and oxidative stress [J].
de Carvalho, Felipe Barbosa ;
de Gomes, Marcelo Gomes ;
Pinto Savall, Anne Suely ;
Fidelis, Eduarda Monteiro ;
Pinton, Simone ;
Funguetto Ribeiro, Ana Claudia ;
Munieweg, Felix Roman ;
Oelke, Carlos Alexandre ;
Haas, Sandra Elisa .
PHARMACOLOGICAL REPORTS, 2021, 73 (02) :563-573
[6]   Curcumin Efficacy in a Serum/Glucose Deprivation-Induced Neuronal PC12 Injury Model [J].
Farkhondeh, Tahereh ;
Ashrafizadeh, Milad ;
Azimi-Nezhad, Mohsen ;
Samini, Fariborz ;
Aschner, Michael ;
Samarghandian, Saeed .
CURRENT MOLECULAR PHARMACOLOGY, 2021, 14 (06) :1146-1155
[7]   Berberine-loaded M2 macrophage-derived exosomes for spinal cord injury therapy [J].
Gao, Zhan-Shan ;
Zhang, Chuan-Jie ;
Xia, Nan ;
Tian, He ;
Li, Dao-Yong ;
Lin, Jia-Quan ;
Mei, Xi-Fan ;
Wu, Chao .
ACTA BIOMATERIALIA, 2021, 126 (126) :211-223
[8]   Effect of main ingredients of Danhong Injection against oxidative stress induced autophagy injury via miR-19a/SIRT1 pathway in endothelial cells [J].
Guo, Yan ;
Yang, Jie-hong ;
Cao, Shi-dong ;
Gao, Cheng-xian ;
He, Yu ;
Wang, Yu ;
Wan, Hai-tong ;
Jin, Bo .
PHYTOMEDICINE, 2021, 83
[9]   Curcumin-laden exosomes target ischemic brain tissue and alleviate cerebral ischemia-reperfusion injury by inhibiting ROS-mediated mitochondrial apoptosis [J].
He, Ruyi ;
Jiang, Yibing ;
Shi, Yijie ;
Liang, Jia ;
Zhao, Liang .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2020, 117
[10]   Resveratrol Enhances Wound Healing in Type 1 Diabetes Mellitus by Promoting the Expression of Extracellular Vesicle-Carried MicroRNA-129 Derived from Mesenchymal Stem Cells [J].
Hu, Jianxia ;
Liu, Xiaoyi ;
Chi, Jingwei ;
Che, Kui ;
Ma, Xiaolong ;
Qiu, Mingyue ;
Fu, Zhengju ;
Wang, Yahao ;
Wang, Yangang ;
Wang, Wei .
JOURNAL OF PROTEOME RESEARCH, 2022, 21 (02) :313-324
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