A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegans

被引:4
作者
Liu, Chenshu [1 ,2 ,3 ]
Rex, Rachel [4 ]
Lung, Zoe [3 ]
Wang, John S. [3 ]
Wu, Fan [3 ]
Kim, Hyung Jun [1 ,2 ]
Zhang, Liangyu [1 ,2 ,3 ]
Sohn, Lydia L. [4 ]
Dernburg, Abby F. [1 ,2 ,3 ,5 ]
机构
[1] Univ Calif Berkeley, Calif Inst Quantitat Biosci QB3, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[3] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA
[4] Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA
[5] Lawrence Berkeley Natl Lab, Dept Biol Sci & Engn, Life Sci Div, Berkeley, CA 94720 USA
关键词
SYNAPSIS; COMPLEX; PROTEINS; LAMINS; ROLES; MEIOSIS; GENOME; MATEFIN/SUN-1; ARCHITECTURE; COMPONENTS;
D O I
10.1126/sciadv.abn5709
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Oogenesis involves transduction of mechanical forces from the cytoskeleton to the nuclear envelope (NE). In Caenorhabditis elegans, oocyte nuclei lacking the single lamin protein LMN-1 are vulnerable to collapse under forces mediated through LINC (linker of nucleoskeleton and cytoskeleton) complexes. Here, we use cytological analysis and in vivo imaging to investigate the balance of forces that drive this collapse and protect oocyte nuclei. We also use a mechano-node-pore sensing device to directly measure the effect of genetic mutations on oocyte nuclear stiffness. We find that nuclear collapse is not a consequence of apoptosis. It is promoted by dynein, which induces polarization of a LINC complex composed of Sad1 and UNC-84 homology 1 (SUN-1) and ZYGote defective 12 (ZYG-12). Lamins contribute to oocyte nuclear stiffness and cooperate with other inner nuclear membrane proteins to distribute LINC complexes and protect nuclei from collapse. We speculate that a similar network may protect oocyte integrity during extended oocyte arrest in mammals.
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页数:14
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