Role of GSDMD and VEGF in differentiating between malignant and non-malignant pleural effusions

被引:0
|
作者
El-Kalashy, Mai Mahmoud [1 ]
Eid, Hanaa Abdelmohsen [1 ]
Awad, Samah Mohammed [2 ]
Allam, Esraa Tawfik [2 ]
Hassan, Reham Ahmed Abdelaziz [1 ]
El-Koa, Amal Ali [1 ]
机构
[1] Menoufia Univ, Fac Med, Menoufia, Egypt
[2] Menoufia Univ, Natl Liver Inst, Menoufia, Egypt
关键词
GSDMD; VEGF; Pleural effusion; ENDOTHELIAL GROWTH-FACTOR; PYROPTOSIS; UTILITY; FLUID;
D O I
10.1186/s43088-023-00366-9
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
BackgroundIt is crucial to differentiate between benign and malignant pleural effusions while making a diagnosis. The purpose of this research was to investigate the diagnostic significance of GSDMD and VEGF in discriminating between different kinds of pleural effusion and their correlation with both progression-free and overall survivals in the malignant type.MethodsNinety-one pleural fluid samples, which were classified as transudates or exudates (tuberculous, para-infectious, or malignant) by pleural fluid classifications, were tested for GSDMD using sandwich ELIZA kits, and 41 of the exudative samples were randomly selected for VEGF testing. Both markers' diagnostic accuracy was assessed.ResultsThe lowest level of GSDMD was associated with the transudate group (mean and SD of 2.35 +/- 0.44 ng/mL) and the highest in the malignant effusion group (mean and SD of 4.38 +/- 1.67 ng/mL). The specificity and sensitivity of GSDMD in the diagnosis of exudative PE were 97% and 98%, respectively (p = 0.001) with the cutoff point = 2.89). Regarding VEGF, its level was 222.3 +/- 53.4 pg/ml for all studied samples where MPE (n = 21) was 261.2 +/- 48.2 pg/ ml (mean +/- SD), TBPE (n = 7) was 185.4 +/- 6.96 pg/ml (mean +/- SD), and PIPE (n = 13) was 179.3 +/- 13.9 pg/ml (mean +/- SD). The diagnostic accuracy of VEGF for the detection of MPE was 90% with a sensitivity of 100% and specificity of 80% and the cutoff point was 191.5 pg/ml. There were highly significant inverse correlations between progression-free survival and both GSDMD (r =- 0.531, p = 0.009) and VEGF (r = - 0.582, p = 0.006) in MPE.ConclusionPleural effusion GSDMD can be an effective marker for differentiating the different kinds of PE, and VEGF levels can be a useful adjuvant marker in screening out MPE as a possible diagnosis, leading to the proper selection of patients who may benefit from more invasive procedures.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] KARYOTYPE ANALYSIS OF CELLS IN MALIGNANT AND NON-MALIGNANT EFFUSIONS
    COUTINHO, V
    BOTTURA, C
    CARYOLOGIA, 1975, 28 (03) : 345 - 358
  • [22] Differentiating between malignant and idiopathic pleural effusions:: the value of diagnostic procedures
    Aleman, C.
    Sanchez, L.
    Alegre, J.
    Ruiz, E.
    Vazquez, A.
    Soriano, T.
    Sarrapio, J.
    Teixidor, J.
    Andreu, J.
    Felip, E.
    Armadans, L.
    De Sevilla, T. Fernandez
    QJM-AN INTERNATIONAL JOURNAL OF MEDICINE, 2007, 100 (06) : 351 - 359
  • [23] Utility of LDH-A in the differentiation between lymphocytic pleural effusions of malignantand non-malignant etiology
    Zivkovic, Nevenka Piskac
    Malnar, Linda
    Kovacevic, Ivona
    EUROPEAN RESPIRATORY JOURNAL, 2020, 56
  • [24] IPC use in non-malignant effusions
    Davidson, Richard
    Aujayeb, Avinash
    Aujayeb, Avinash
    EUROPEAN RESPIRATORY JOURNAL, 2021, 58
  • [25] VEGF A, C, and D levels in malignant pleural effusions
    Croghan, G. A.
    Nichols, F.
    Cassivi, S.
    Nevala, W.
    Daniels, C.
    Thompson, M.
    Schroeder, D.
    Markovic, S. N.
    JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (15)
  • [26] Defining the cellular composition and associated molecular phenotypes of malignant and non-malignant cells in breast cancer pleural effusions
    Whitfield, Holly J.
    Berthelet, Jean
    Mangiola, Stefano
    Bell, Caroline
    Anderson, Robin L.
    Pal, Bhupinder
    Papenfuss, Anthony T.
    Yeo, Belinda
    Merino, Delphine
    Davis, Melissa J.
    CANCER RESEARCH, 2022, 82 (12)
  • [27] NON-MALIGNANT ASBESTOS PLEURAL DISEASE
    HILLERDAL, G
    THORAX, 1981, 36 (09) : 669 - 675
  • [28] Role of vascular endothelial growth factor (VEGF) in the diagnosis of malignant pleural effusions
    Behera, D
    Reddy, C
    Dash, S
    LUNG CANCER, 2005, 49 : S116 - S116
  • [29] NON-MALIGNANT PLEURAL DISEASE AND ASBESTOS
    SMITH, WHR
    DAVIES, D
    BRITISH JOURNAL OF DISEASES OF THE CHEST, 1980, 74 (04): : 418 - 418
  • [30] Extracellular Vesicle MicroRNAs Can Differentiate Between Malignant and Non-malignant Pleural Effusion
    Shojaee, S.
    Romano, G.
    Nigita, G.
    Fadda, P.
    Yermakhanova, G.
    Sanchez, T. M.
    Saviana, M.
    Calore, F.
    Zou, Y.
    Robila, V.
    Lentz, R. J.
    Grogan, E.
    Deppen, S.
    Maldonado, F.
    Chen, H.
    Rahman, N. M.
    Acunzo, M.
    Pua, H.
    Blackwell, T. S.
    Nana-Sinkam, P.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2024, 209