A rapid and simple method for simultaneous determination of three breast cancer related microRNAs based on magnetic nanoparticles modified with S9.6 antibody

被引：5

作者：

Ghanbari, Reza ^{[1
]}

Isfahani, Ali Attaripour ^{[1
]}

Pirmoradian, Sina ^{[1
]}

Rezaei, Halimeh ^{[2
]}

Radfar, Sasan ^{[3
]}

Kheirollahi, Majid ^{[4
]}

机构：

[1] Islamic Azad Univ, Dept Biol Sci & Technol, Najafabad Branch, Najafabad, Iran

[2] Univ Isfahan, Fac Sci, Biol Dept, Genet Div, Esfahan 8174673441, Iran

[3] Univ Tehran Med Sci, Stem Cell & Regenerat Med Ctr Excellence, Tehran, Iran

[4] Isfahan Univ Med Sci, Sch Med, Dept Genet & Mol Biol, Esfahan, Iran

来源：

ANALYTICAL BIOCHEMISTRY | 2023年 / 665卷

关键词：

microRNAs; Breast cancer; Titanium phosphate; Electrochemical biosensor; Magnetic nanoparticles; ELECTROCHEMICAL IMMUNOSENSOR; ULTRASENSITIVE DETECTION; OVEREXPRESSION; EXPRESSION; BIOSENSOR; PLATFORM; MIR-155; MIR-21; SERUM;

D O I：

10.1016/j.ab.2023.115052

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cancer progression is typically associated with the simultaneous changes of multiple microRNA (miR) levels. Therefore, simultaneous determination of multiple miR biomarkers exhibits great promise in early diagnosis of cancers. This research seeks to discuss a simple biosensing method for the ultrasensitive and specific detection of the three miRs related to the breast cancer based on S9.6 antibody coated magnetic beads, titanium phosphate nanospheres, and screen-printed carbon electrode. To prepare signaling probes, three hairpin DNAs (hDNAs) were labeled with three encoding titanium phosphate nanospheres with large quantities of different heavy metal ions (zinc, cadmium, lead), which have been utilized to discriminate the signals of three microRNA targets in relation with the corresponding heavy metal ions. After that, these hairpin structures hybridize with miR-21, miR-155 and miR-10b to form miR-21/hDNA1, miR-155/hDNA2 and miR-10b/hDNA3 complexes, which were captured by S9.6 antibodies (one anti-DNA/RNA antibody) pre-modified on magnetic bead surface. Therefore, the specific preconcentration of targets from complex matrixes can be carried out using magnetic actuation, increasing the sensitivity and specificity of the detection. The biosensor was suitably applied for direct and rapid detection of multiple microRNAs in real sample. It was observed that there were no significant differences between the results obtained by the suggested method and qRT-PCR as a reference method. So, this method makes an ultrasensitive novel platform for miRNAs expression profiling in clinical diagnosis and biomedical research.

引用

页数：10