Isolation and Functional Analysis of Na+/H+ Antiporter Gene (LcNHX1) from Leymus chinensis

被引：1

作者：

Sun, Chuanbo ^{[1
]}

Zhang, Chuang ^{[1
]}

Yin, Yuejia ^{[1
]}

Wang, Ying ^{[2
]}

Mu, Shujing ^{[1
]}

Liu, Qing ^{[1
]}

Wang, Ziyu ^{[1
]}

Liu, Yang ^{[1
]}

Zhang, Chunxiao ^{[1
]}

Liu, Xiangguo ^{[1
]}

Zhang, Yan ^{[1
]}

Guo, Jia ^{[1
]}

机构：

[1] Jilin Acad Agr Sci, Changchun 130033, Peoples R China

[2] Changchun Vocat Inst Technol, Dept Food & Biol, Changchun 130033, Peoples R China

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2024年 / 42卷 / 04期

关键词：

NHX1; Leymus chinensis; Saline-alkali tolerance; Candidate gene; CONFERS SALT TOLERANCE; ARABIDOPSIS-THALIANA; STRESS TOLERANCE; EXPRESSION; PLANT; CLONING; OVEREXPRESSION; EXCHANGER; IMPROVES; ALKALI;

D O I：

10.1007/s11105-024-01446-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Saline-alkali stress has adverse effects on plant growth. Some plant Na+/H+ antiporters were reported to be important in salt tolerance. However, it needs to be better understood that Na+/H+ antiporters are involved in plant salt-alkali (NaHCO3/Na2CO3) tolerance. In this study, a Na+/H+ antiporter gene LcNHX1 (China patent No.200810050629.1) has been cloned from Leymus chinensis. The LcNHX1 CDS contains 1614 bp that encodes 537 amino acids. Amino acid and nucleotide sequence similarity, protein topology modelling, conserved functional domains in the protein sequence, and subcellular localization classified LcNHX1 as a vacuolar NHX1 homolog. Transcription analysis by quantitative RT-PCR indicated that upregulated expression of LcNHX1 could be induced by NaCl, NaHCO3, NaCl + NaHCO3, and PGE in L. chinensis seedlings. The expression of LcNHX1 partially complements the salt-sensitive phenotypes of a Delta nhx1 yeast strain. In addition, LcNHX1 overexpressing enhanced the tolerance to NaHCO3 stress in the transgenic Arabidopsis. Taken together, these results indicated that LcNHX1 is a potential candidate gene for enhancing plant saline-alkali tolerance.

引用

页码：665 / 674

页数：10

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