Development of a Validated RP-HPLC Method for the Estimation of Curcumin in Nanoemulsion and in Its Phase Solubility Studies

被引：1
作者：
Naz, Zrien [1 ,2 ]
Faisal, Mohammad Shah [2 ]
Khan, Aquib Belal [1 ]
Naz, Afrien [1 ]
Ahmad, Farhan Jalees [1 ]
机构：
[1] Jamia Hamdard, New Delhi, India
[2] Al Asmarya Univ, Coll Pharm, Zliten, Libya
来源：
JOURNAL OF APPLIED SPECTROSCOPY | 2024年 / 90卷 / 06期
关键词：
RP-HPLC; curcumin; phase solubility; nanoemulsion; NF-KAPPA-B; EXPRESSION; ACTIVATION; MECHANISM; ALPHA;
D O I：
10.1007/s10812-024-01678-z
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The study developed a simple, sensitive, and robust method for the estimation of curcumin in bulk drug form and in nanoemulsion formulation. Mobile phase consisting of acetonitrile and 20 mM potassium dihydrogen orthophosphate (pH 3.0) in ratio 55:45 was selected. A flow rate of 1 mL/min and an injection volume of 20 mu L was injected to develop a chromatogram using a 516 C18 DB reversed-phase column. The detection wavelength was adjusted at 420 nm and the retention time of curcumin was found to be 9.78 +/- 0.5 min. The method was validated as per ICH guidelines and can be used for the quantification of curcumin even at a very low concentration.
引用
收藏
页码：1404 / 1410
页数：7
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