Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia

被引：17

作者：

Lim, Younggap ^{[1
]}

Kim, Hyun Young ^{[1
,2
]}

Han, Dohyun ^{[3
,4
,5
,7
]}

Choi, Bong-Kyu ^{[1
,6
]}

机构：

[1] Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea

[2] Seoul Natl Univ, Dent Res Inst, Sch Dent, Seoul, South Korea

[3] Seoul Natl Univ Hosp, Transdisciplinary Dept Med & Adv Technol, Seoul, South Korea

[4] Seoul Natl Univ Hosp, Biomed Res Inst, Prote Core Facil, Seoul, South Korea

[5] Seoul Natl Univ, Dept Med, Coll Med, Seoul, South Korea

[6] Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, 101 Daehak Ro, Seoul 03080, South Korea

[7] Seoul Natl Univ Hosp, Transdisciplinary Dept Med & Adv Technol, 101 Daehak Ro, Seoul 03080, South Korea

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2023年 / 12卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

extracellular vesicles; macrophages; periodontitis; proteome; Tannerella forsythia; OUTER-MEMBRANE VESICLES; IDENTIFICATION; BIOGENESIS; MECHANISMS; TISSUE;

D O I：

10.1002/jev2.12381

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Periodontitis is a chronic inflammatory disease caused by periodontal pathogens in subgingival plaque and is associated with systemic inflammatory diseases. Extracellular vesicles (EVs) released from host cells and pathogens carry a variety of biological molecules and are of interest for their role in disease progression and as diagnostic markers. In the present study, we analysed the proteome and inflammatory response of EVs derived from macrophages infected with Tannerella forsythia, a periodontal pathogen. The EVs isolated from the cell conditioned medium of T. forsythia-infected macrophages were divided into two distinct vesicles, macrophage-derived EVs and T. forsythia-derived OMVs, by size exclusion chromatography combined with density gradient ultracentrifugation. Proteome analysis showed that in T. forsythia infection, macrophage-derived EVs were enriched with pro-inflammatory cytokines and inflammatory mediators associated with periodontitis progression. T. forsythia-derived OMVs harboured several known virulence factors, including BspA, sialidase, GroEL and various bacterial lipoproteins. T. forsythia-derived OMVs induced pro-inflammatory responses via TLR2 activation. In addition, we demonstrated that T. forsythia actively released OMVs when T. forsythia encountered macrophage-derived soluble molecules. Taken together, our results provide insight into the characterisation of EVs derived from cells infected with a periodontal pathogen.

引用

页数：35

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